Study on the Digestibility and Interaction of Quercetin and Rutin to Wheat Starch
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摘要: 本文通过模拟小麦淀粉的体外消化实验研究了槲皮素和芦丁与小麦淀粉复合后的消化特性差异，并通过淀粉与碘的显色反应、红外光谱和X-射线衍射等试验研究槲皮素和芦丁与小麦淀粉的相互作用。结果表明：在槲皮素和芦丁与小麦淀粉的混合体系中，随着老化时间的延长，快消化淀粉显著减少（P<0.05），慢消化淀粉和抗性淀粉显著增加（P<0.05）。槲皮素和芦丁的添加，对淀粉葡萄糖苷酶和α-淀粉酶的活性均有抑制作用。槲皮素或芦丁与小麦淀粉之间存在相互作用，并通过氢键或疏水键相互结合。槲皮素和芦丁对酶的抑制和与小麦淀粉之间的相互作用，降低了小麦淀粉的消化性，增加淀粉的抗消化性，为开发降血糖等相关功能食品的开发奠定了理论基础。Abstract: In this paper,the in vitro digestibility of wheat starch compounded with quercetin and rutin was studied by simulating the in vitro digestibility of wheat starch,the interaction of quercetin and rutin with wheat starch was studied by the color reaction of starch and iodine,infrared spectrum and X-ray diffraction. The results showed that in the mixed system of quercetin,rutin and wheat starch,the fast digestible starch decreased significantly(P<0.05)and the slow digestible starch and resistant starch increased significantly with the aging time(P<0.05). Quercetin and rutin inhibited the activities of starch glucosidase and alpha-amylase.The interaction between quercetin or rutin and wheat starch was confirmed,and they were bound by hydrogen bond or hydrophobic bond. However,the inhibition of quercetin and rutin on enzyme and the interaction with wheat starch reduced the digestibility of wheat starch and increased the resistant digestibility of wheat starch,which laid a theoretical foundation for the development of functional food such as hypoglycemic food.
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Keywords:
- quercetin /
- rutin /
- wheat starch /
- digestibility /
- interaction
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[1] Englyst H N,Kingman S M,Cummings J H. Classification and measurement of nutritionally important starch fractions[J]. European Journal of Clinical Nutrition,1992,46(Suppl 2):S33.
[2] Englyst H N,Hudson G J. The classification and measurement of dietary carbohydrates[J]. Food Chemistry,1996,57(1):15-21.
[3] 姚莉萍,刘振东,张文会,等. 糌粑体外模拟消化研究[J]. 高原农业. 2020,4(2):172-177. [4] Camelo-Méndez G A,Agama-Acevedo E,Sanchez-Rivera M M,et al. Effect on in vitro starch digestibility of mexican blue maize anthocyanins[J]. Food Chemistry,2016,211(15):281-284.
[5] Mkandawire N L,Kaufman R C,Bean S R,et al. Effects of sorghum(Sorghum bicolor(l.)moench)tannins on α-amylase activity and in vitro digestibility of starch in raw and processed flours[J]. Journal of Agricultural & Food Chemistry,2013,61(18):4448-4454.
[6] 朱建宇,齐宝坤,李杨,等. 生物酶法豆渣对淀粉理化性质及饼干消化特性的影响[J]. 食品工业科技,2019,40(19):13-17 ,23.
[7] 孔露,孔茂竹,余佳熹,等. 藜麦淀粉消化特性与理化特性研究[J]. 食品科技,2019,44(4):285-290. [8] 李勇,周文化,李彦,等. 板栗鲜湿面体外淀粉消化特性研究[J]. 食品科技,2019,44(10):165-169. [9] 靳志强,陈艳彬,王玺. 湿热处理和发酵对小米营养素生物可给性及淀粉消化性的影响[J]. 中国食品学报,2019,19(12):177-186. [10] Lemlioglu-Austin D,Turner N D,Mcdonough C M,et al. Effects of sorghum[Sorghum bicolor(L.)Moench]crude extracts on starch digestibility,estimated glycemic index(EGI),and resistant starch(Rs)contents of porridges[J]. Molecules,2012,17(9):11-24.
[11] 李铁梅,王玺,段盛林,等. 不同熟化处理方式及多酚类物质对马铃薯全粉消化特性的影响[J]. 食品研究与开发,2020,41(8):1-7. [12] 沈威. 柑橘黄酮对血糖的调节及其作用机制研究[D]. 上海:华东理工大学,2013. [13] 王中荣. 不同直链淀粉含量的玉米淀粉理化性质及其应用研究[D]. 重庆:西南大学,2007. [14] 柴艳伟. 茶多酚对高直链玉米淀粉消化性的影响及机理[D].无锡:江南大学,2013. [15] 赵蓓蓓,贾祥泽,孙思薇,等. 淀粉-多酚复合物理化及功能性的研究进展[J]. 食品科学,2018,39(13):297-303. [16] Hyun-Jung Chung,Seung-Taik Lim,Dong-Hoon Shin. In vitro starch digestibility and estimated glycemic index of chemically modified corn starches[J]. Food Research International,2008,41(6):579-584.
[17] He Q,Lv Y,Yao K. Effects of tea polyphenols on the activities of α-amylase,pepsin,trypsin and lipase[J]. Food Chemistry,2007,101(3):1178-1182.
[18] Barrett A H,Farhadi N F,Smith T J. Slowing starch digestion and inhibiting digestive enzyme activity using plant flavanols/tannins-a review of efficacy and mechanisms[J]. LWT-Food Science and Technology,2017,87:394-399.
[19] 于轩. 不同来源淀粉的分子结构对其酶解性能影响的研究[D].无锡:江南大学,2013. [20] Tester R F,Qi J K X. Starch structure and digestibility enzyme-substrate relationship[J]. Worlds Poultry Science Journal,2004,60(2):186-195.
[21] 李欢欢,吴娜娜,谭斌,等. 黑米米糠游离酚对碘与淀粉结合及淀粉消化的影响[J]. 食品科学技术学报,2017,30(5):18-24. [22] Shen W,Xu Y,Lu Y H. Inhibitory effects of citrus flavonoids on starch digestion and antihyperglycemic effects in HepG2 cells[J]. Journal of Agricultural & Food Chemistry,2012,60(38):9609-9619.
[23] Goodfellow B J,Wilson R H. A fourier transform IR study of the gelation of amylose and amylopectin[J]. Biopolymers,1990,30(13-14):1183-1189.
[24] Lopez-Rubio A,Flanagan B M,Shrestha A K,et al. Molecular rearrangement of starch during in vitro digestion:Toward a better understanding of enzyme resistant starch formation in processed starches[J]. Biomacromolecules,2008,9(7):1951-1958.
[25] Wu Y,Chen Z X,Li X X,et al. Effect of tea polyphenols on the retrogradation of rice starch[J]. Food Research International,2009,42(2):221-225.
[26] Wu Y,Lin Q,Chen Z,et al. The interaction between tea polyphenols and rice starch during gelatinization[J]. Food Science and Technology International,2011,17(6):569.
[27] 屠一峰.现代仪器分析[M]. 北京:科学出版社,2011. [28] Zobel H F,Young S N,Rocca L A. Starch gelatinization:An X-ray diffraction study[J]. Cereal Chemistry,1988,65(6):647-666.
[29] 陈佩. 不同链/支比玉米淀粉的形态及其在有/无剪切力下糊化的研究[D].广州:华南理工大学,2010. [30] Li W,Shan Y,Xiao X,et al. Physicochemical properties of A-and B-starch granules isolated from hard red and soft red winter wheat[J]. Journal of Agricultural & Food Chemistry,2013,61(26):6477-6484.
[31] Lian X,Kang H,Sun H,et al. Identification of the main retrogradation-related properties of rice starch[J]. Journal of Agricultural & Food Chemistry,2015,63(5):1562-1572.
[32] Ziobro R,Korus J,Juszczak L,et al. Influence of inulin on physical characteristics and staling rate of gluten-free bread[J]. Journal of Food Engineering,2013,116(1):21-27.
[33] 沙晨希,田耀旗,金征宇. V-Ⅰ-型慢消化淀粉的制备与鉴定[J]. 食品与机械,2013(3):37-39. -
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