Abstract: The method of using QuEChERS kit in combination with high performance liquid chromatography for determination of arsanilic acid and roxarsone in eggs was established. The sample was mixed and extracted with methanol solution (1:1, V/V) of acetonitrile and 10% trifluoroacetic acid, centrifuged, and the supernatant was taken and purified in QuEChERS purification tube (containing purification packing 400 mg PSA, 400 mg C₁₈ EC, 45 mg GCB, 1199 mg MgSO₄), and then detected by HPLC.The results showed that there was a good linear relationship between arsanilic acid and roxarsone in the range of 1.00～100.00 mg/L, and the correlation coefficients were all greater than 0.999.The detection limits were 0.044 and 0.063 mg/kg, respectively.The quantitative limits were 0.149 and 0.211 mg/kg, respectively. The recovery rate of arsanilic acid was 92.32%～95.95%, and the relative standard deviation was 1.54%～4.92%. The recovery rate of roxarsone were 89.84%～94.91%, and the relative standard deviation was 2.19%～5.87%.The method has high accuracy, good precision, simple and rapid, and is suitable for the determination of organoarsenic veterinary drug residues in eggs.This study provides a method basis for trace analysis of banned organic arsenic preparations in animal-derived foods.