樟树精油及其单体抗白色念珠菌活性及安全性研究

孙伟杰; 雷博; 何园; 马淑梅; 刘楠; 梅其炳; 刘莉

doi:10.13386/j.issn1002-0306.2020050166

樟树精油及其单体抗白色念珠菌活性及安全性研究

孙伟杰^1,2,
雷博²,
何园²,
马淑梅²,
刘楠²,
梅其炳²,
刘莉^1,2, ,

1. 西南医科大学药学院, 四川泸州 646000;
2. 中国医药工业研究总院药理评价研究中心, 上海 200040

详细信息

作者简介:
孙伟杰(1995-),男,硕士研究生,研究方向:中药药理与分子药理,E-mail:545807134@qq.com。

通讯作者:
刘莉(1967-),女,博士,研究员,研究方向:中药药理与分子药理,E-mail:1433562847@qq.com。

中图分类号: TS201.6
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出版历程
- 收稿日期: 2020-05-14
- 网络出版日期: 2021-01-20
- 刊出日期: 2021-01-14

Anti-Candida albicans Activity and Safety of Cinnamomum camphora Essential Oil and Its Components

SUN Weijie^1,2,
LEI Bo²,
HE Yuan²,
MA Shumei²,
LIU Nan²,
MEI Qibing²,
LIU Li^1,2, ,

1. Pharmacy of Southwestern Medical University School, Luzhou 646000, China;
2. Center for Pharmacology Evaluation and Research, China National Institute of Pharmaceutical Industry, Shanghai 200040, China

摘要

摘要: 为探究樟树精油及其单体抗白色念珠菌活性及安全性，本研究通过纸片法和微量肉汤稀释法测定了樟树精油、葡萄柚精油、柠檬精油、薰衣草精油抗白色念珠菌活性，同时对樟树精油进行GC-MS挥发性成分鉴定，进一步分析樟树精油主要化学成分对白色念珠菌的抑菌活性，最后观察樟树精油及其单体对豚鼠皮肤刺激和小鼠血管收缩性影响。结果表明，樟树精油抗白色念珠菌活性强于葡萄柚、柠檬、薰衣草精油；樟树精油抗白色念珠菌的活性来源于α-松油醇、异松油烯、α-松油烯、松油烯-4-醇、桧烯、1，8-桉叶油素；豚鼠皮肤在连续5 d涂抹4%樟树精油后出现轻微的红斑，而4%松油烯-4-醇在涂抹2 d后就会出现轻微的红斑；另外，4%松油烯-4-醇会明显增加小鼠的血管通透性。研究结果为樟树精油的开发利用及治疗白色念珠菌相关疾病提供理论依据。
- 樟树精油 /
- 白色念珠菌 /
- 单体 /
- 安全性 /
- 血管通透性
Abstract: To explore the anti-Candida albicans activity and safety of Cinnamomum camphora essential oil and its components,the anti-Candida albicans activity of Cinnamomum camphora essential oil that compared with grapefruit,lemon and lavender was determined by disk diffusion method and dilution method. Meanwhile,the GC-MS was used to detect the volatile components of essential oil. Moreover,the significant difference analysis among the chemical components and its correlation with anti-Candida acbicans activity were conducted. Finally,the Cinnamomum camphora essential oil and its components to stimulate the skin of guinea pigs and vascular permeability were observed. The results showed that the anti-Candida albicans activity of Cinnamomum camphora essential oil was stronger than that of grapefruit,lemon and lavender essential oil and it was mainly derived from α-Terpineol,Terpinolene,α-Terpinene,Terpinen-4-ol,Sabinene,1,8-Eucalyptol. The skin of guinea pigs showed slight erythema after 5 days of continuous application of 4% Cinnamomum camphora essential oil,while 4% terpinen-4-ol showed slight erythema after 2 days of application. In addition,4% terpinene-4-ol could significantly increase the vascular permeability of mice. The results would provide a theoretical basis for the development and utilization of Cinnamomum camphora essential oil and the treatment of Candida albicans-related diseases.
- Cinnamomum camphora essential oil /
- Candida albicans /
- component /
- safety /
- vascular permeability

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