Optimization of Microwave-assisted Extraction of Anthocyanins from Adzuki Bean Seed Coat by Response Surface Methodology and Its Stability
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摘要: 以红小豆种皮为试验原料,采用溶剂法、微波法、超声波法、酶法等组合工艺对红小豆种皮花色苷进行提取,并在单因素实验的基础上,应用响应面分析优化了最佳提取工艺条件。同时,研究了pH、光照、温度、食品添加剂、金属离子对红小豆种皮花色苷稳定性的影响。结果表明:红小豆花色苷的最佳提取方法为微波辅助溶剂提取法,各因素对花色苷提取效果影响的主次顺序为料液比>微波功率>微波时间,最佳提取工艺条件为:料液比1:30(g/mL),微波功率463 W、微波时间42 min,在此条件下得出的红小豆种皮花色苷含量为32.08 mg/100 g。红小豆种皮花色苷在pH<5的酸性条件下稳定性较好,在光照时间大于2 d和温度高于80℃的条件下稳定性差,食品添加剂蔗糖、葡萄糖、氯化钠和苯甲酸钠对其无明显影响,添加柠檬酸对其有增色作用,在Na+、Mg2+、K+离子溶液中稳定性好,在Fe3+、Cu2+离子溶液中稳定性较差。Abstract: The adzuki bean seed skin was taken as testing material to extract anthocyanins. The solvent extraction method, microwave extraction method, ultrasonic extraction method, enzymatic extraction method and other combined method processes were investigated. On the basis of single factor test, the response surface analysis was used to optimize the best extraction protocols and experimental conditions. At the same time, the effect of pH, light, temperature, food additives, and metal ions on the stability of anthocyanin in adzuki bean seed skin was studied. The results showed that the best extraction method was microwave-assisted solvent extraction, the impact of various factors on effect of anthocyanins extraction primary and secondary order of material-liquid ratio > microwave power > microwave time, the best extraction conditions were: Material-liquid ratio 1:30 (g/mL), microwave power 463 W, microwave time 42 min, the anthocyanin content of the adzuki bean seed skin obtained under this condition was 32.08 mg/100 g. Anthocyanins from adzuki bean seed skin was stable under acidic conditions with pH<5, but poor stability under conditions of light time greater than two days and temperature higher than 80℃. Food additives sucrose, glucose, sodium chloride and sodium benzoate had no obvious effect on its stability, and the addition of citric acid will increase its color function, it showed better stability in Na+, Mg2+, K+ion solution than that in Fe3+, Cu2+ ion solution.
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Keywords:
- Adzuki bean /
- anthocyanins /
- pigment /
- microwave extraction /
- response surface analysis /
- stability
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[1] 蒋晓春,雷广军,艾米如拉·图尔贡,等.红黑小豆杂交F3世代粒色变异和抗氧化性分析[J].北京农学院学报,2015,30(3):1-5. [2] 郭兆萍,王彩萍,赵吉平,等.试论红小豆的经济价值及产业化开发利用[J].农业科技通讯,2015(7):10-12. [3] 李洋,韩小贤,成军虎,等.基于正交试验法优化红小豆色素提取条件研究[J].农产品加工(学刊),2011(7):23-25. [4] 韩涛,甘育新,李丽萍,等.红小豆种皮红色素的提取及其理化性质的研究[J].中国粮油学报,1997,12(6):58-62 [5] 刘雪雁,马爱红,杨昌群,等.红豆皮提取天然色素的实验室研究[J].吉林工学院学报(自然科学版),1999,20(2):3-5. [6] 李皓雲,段俊丽,王一尘.原花青素的功能与研究进展[J].国际老年医学杂志,2013,34(2):73-76. [7] Stevens L J,Kuczek T,Burgess J R,et al. Mechanisms of behavioral,atopic,and other reactions to artificial food colors in children[J].Nutrition Reviews,2013,71(5):268-281.
[8] 李京城,张唐伟,兰小中,等.花色苷的生物活性及提取、分离纯化研究[J]. 安徽农学通报,2020,26(13):140-144. [9] Belkacemi A,Ramassamy C.Anthocyanins protect SK-N-SH cells against acrolein-induced toxicity by preserving the cellular redox state[J].Journal of Alzheimer's Disease,2016,50(4):981-998.
[10] Pergola C,Rossi A,Dugo P,et al. Inhibition of nitric oxide biosynthesis by anthocyanin fraction of blackberry extract[J].Nitric Oxide,2006,15(1):30-39.
[11] Nankar A N,Dungan B,Paz N,et al. Quantitative and qualitative evaluation of kernel anthocyanins from southwestern United States blue corn[J].Journal of the Science of Food and Agriculture,2016,96(13):4542-4552.
[12] 沈静,徐楠,姚军,等.新疆野生樱桃李果皮中花色苷提取工艺优化及成分鉴定[J].食品安全质量检测学报,2019,10(19):6615-6621. [13] 崔京燕. 树莓果实成熟过程中多酚类化合物的变化及其提取和应用[D]. 太原:中北大学,2019. [14] 薛宏坤,刘成海,刘钗,等.响应面法和遗传算法-神经网络模型优化微波萃取蓝莓中花青素工艺[J].食品科学,2018,39(16):280-288. [15] 李双石,李浡,张虎成,等.超声波提取条件对酿酒葡萄皮渣花色苷的影响[J].食品工业科技,2013,34(18):294-297. [16] Lotfi L,Kalbasi-Ashtari A,Hamedi M,et al. Effects of enzymatic extraction on anthocyanins yield of saffron tepals(Crocos sativus)along with its color properties and structural stability[J].Journal of Food and Drug Analysis,2015,23(2):210-218.
[17] 王心哲,孟祥敏,游颖,等.微波辅助水提法提取黑米花色苷的工艺研究[J].粮食与油脂,2020,33(1):94-96. [18] 姜龙云,杨春权,杨晓娜.微波辅助提取红花羊蹄甲花色苷的工艺研究[J].南方农业,2019,13(27):131-134. [19] 潘钊言,张镖,冯海锜,等.微波提取玫瑰茄花色苷类色素工艺研究[J].广东化工,2019,46(12):31-32. [20] Liu C H,Xue H K,Shen L Y,et al. Improvement of anthocyanins rate of blueberry powder under variable power of microwave extraction[J].Separation and Purification Technology,2019,226:286-298.
[21] Zheng X Z,Xu X W,Liu C H,et al. Extraction characteristics and optimal parameters of anthocyanin from blueberry powder under microwave-assisted extraction conditions[J].Separation and Purification Technology,2013,104:17-25.
[22] 孔茜,陈小全,张燕,等.超声波作用下提取红豆皮色素及其稳定性试验[J].中国调味品,2012,37(12):45-49. [23] 宋德群,孟宪军,王晨阳,等.蓝莓花色苷的pH示差法测定[J].沈阳农业大学学报,2013,44(2):231-233. [24] 韩富亮,李杨,李记明,等.红葡萄酒花色苷结构和颜色的关系研究进展[J].食品与生物技术学报,2011,30(3):328-336. [25] 汪建红.荸荠皮中色素的提取工艺优化[J].食品研究与开发,2019,40(8):164-168. [26] 韦红边,小兰,张明生,等. 金铁锁愈伤组织花色苷的稳定性研究[J]. 山地农业生物学报,2016,35(2):7-14. [27] 蒋海伟,杨婷婷,李佳桥,等.响应面法优化红米花色苷微波辅助提取工艺及其抗氧化活性研究[J].中国食品学报,2015,15(5):74-81. [28] 徐菀璐,罗华,来明月,等.超声波辅助提取玫瑰花色素及其稳定性研究[J].食品安全质量检测学报,2018,9(11):2830-2835. [29] Laleh G H,Frydoonfar H,Heidary R,et al. The effect of light,temperature,pH and species on stability of anthocyanin pigments in four Berberis species[J].Pakistan Journal of Nutrition,2005,5(1):90-92.
[30] 田密霞,胡文忠,李亚东,等.不同品种蓝莓花色苷稳定性的比较研究[J].食品工业科技,2015,36(13):60-65 ,71.
[31] 陈凌,骆卢佳,曹巧巧,等.马齿苋花色苷的稳定性分析[J].现代食品科技,2019,35(1):227-232. [32] 罗桂杰,刘博,陈芬.宿晓红葡萄色素提取及稳定性研究[J].北方农业学报,2019,47(2):104-110. [33] 冯靖,时玉正,邱晓,等.葡萄皮天然色素的提取工艺及其稳定性研究[J].食品研究与开发,2018,39(4):60-68. [34] 李玲,郭丽萍.超声辅助提取火龙果果皮花色苷及稳定性研究[J].食品工业科技,2017,38(6):298-303 ,323.
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