Abstract: Objective:To investigate the effect of deodorized Triglyceride type-Docosahexaenoic acid(TG-DHA)fish oil on lipid metabolism in mice fed with high-fat diet. Methods:The deodorized TG-DHA fish oil was prepared by liposome embedding technology. Male C57BL/6J mice(6 weeks old)were housed and randomly divided into control group(C),model group(M),TG-DHA fish oil group(O-DHA),deodorized TG-DHA fish oil group(L-DHA).After 8 weeks,blood and liver were collected for lipid accumulation analysis. For gene expression mRNA was extracted from liver for fatty acid synthase(FAS),sterol regulatory element binding protein 1c(SREBP 1c),carnitine palmityl transferase 1(CPt1),lipoprotein lipase(LPL). Results:Liposome embedding could mask the fishy odour in fish oil such as hexanal,2,4-heptadienal. The content of DHA was 73.74%,the particle size was 159.50 nm,and the zeta potential was-43.10 mV. The liposome was stable and easy to be digested and absorbed in animals. L-DHA significantly improved the serum and hepatic lipid levels in mice fed with a high-fat diet(P<0.05),O-DHA significantly improved the serum level and liver TG content of mice fed with high-fat diet(P<0.05). Both of them significantly decreased FAS mRNA expression(P<0.01),and significantly increased CPT1 mRNA expression(P<0.05). The effects of L-DHA seemed to be better. Conclusion:Deodorized TG-DHA fish oil can improve lipid metabolism by high-fat diet. The mechanism is related to the down-regulation of liver fatty acid synthesis and the promotion of fatty acid catabolism.