微波强化壳聚糖固相酸降解研究

许磊; 焦思明; 张文昌; 程功; 孙家言; 赵岩; 张劲松

doi:10.13386/j.issn1002-0306.2020040305

微波强化壳聚糖固相酸降解研究

1. 中国科学院金属研究所沈阳材料科学国家研究中心, 辽宁沈阳 110016;
2. 中国科学院过程工程研究所生化工程国家重点实验室, 北京 100190

基金项目:

国家重点研发计划项目（2016YFB0301502）；国家自然科学基金联合基金项目（U1608255）。

详细信息

作者简介:
许磊(1982-),男,硕士,工程师,研究方向:多糖基生物材料,E-mail:xulei@imr.ac.cn。

通讯作者:
赵岩(1970-),男,博士,研究员,研究方向:多糖基生物材料,E-mail:yanzhao@imr.ac.cn。

张劲松(1963-),男,博士,研究员,研究方向:材料工程,E-mail:jshzhang@imr.ac.cn

中图分类号: TS245.9
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出版历程
- 收稿日期: 2020-04-25
- 网络出版日期: 2021-01-20
- 刊出日期: 2021-01-14

Study of Microwave Enhanced Solid-state Acid Degradation of Chitosan

1. Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China;
2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

摘要

摘要: 本文研究了微波强化下的壳聚糖固相酸降解反应，分析了降解过程中微波辐射功率和盐酸用量等参数对降解产物分子量变化的影响，并利用红外光谱和核磁共振氢谱对降解产物的结构进行了表征。研究表明，微波辐射功率和盐酸用量的增加均有利于壳聚糖分子量的降低。采用微波辐射壳聚糖固相酸化物料15 min，即可获得重均分子量低于50000的低分子量壳聚糖，且糖单元的结构在反应过程中保持稳定。采用微波强化固相酸降解和酶降解复合工艺制备壳寡糖，壳寡糖单位时间内的产能可提高4倍以上。综上所述，本研究建立了一种基于微波强化的壳聚糖固相酸降解的高效制备低分子量壳聚糖和壳寡糖的方法，有助于低分子量壳聚糖和壳寡糖的广泛应用。
- 壳聚糖 /
- 微波强化 /
- 固相酸降解 /
- 壳寡糖 /
- 酶降解
Abstract: Microwave enhanced solid-state acid degradation of chitosan was studied in this paper,the effects of microwave radiation power and hydrochloric acid dosage on the molecular weight of degraded products were analyzed,and their structure was characterized by infrared spectrum and proton nuclear magnetic resonance. The results showed that the increase of microwave radiation power and hydrochloric acid dosage would lead to decreased molecular weight. Low molecular weight chitosan with weight average molecular weight less than 50000 could be obtained by irradiating the solid-state acidification material under microwave for 15 minutes,and the structure of sugar unit remained stable during the reaction. The production capacity of chitooligosaccharide could be increased more than 4 times in unit time by the combination of microwave enhanced solid-state acid degradation and enzyme degradation. In conclusion,this study provided an efficient preparation method of low molecular weight chitosan and chitooligosaccharides based on microwave enhanced solid-state acid degradation technology,which laid a foundation for their various applications.
- chitosan /
- microwave enhancement /
- solid-state acid degradation /
- chitooligosaccharides /
- enzyme degradation

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