景参片的安全性评价及其抗急性酒精性肝损伤作用研究

卢美彤; 孙敬蒙; 房辰晨; 张炜煜

doi:10.13386/j.issn1002-0306.2020040286

景参片的安全性评价及其抗急性酒精性肝损伤作用研究

1. 长春中医药大学药学院, 吉林长春 130117;
2. 吉林大学白求恩第一医院药学部, 吉林长春 130021

基金项目:

吉林省科技厅项目（201603089YY）。

详细信息

作者简介:
卢美彤(1996-),女,硕士研究生,研究方向:药物新剂型与新技术研究,E-mail:1326227595@qq.com。

通讯作者:
张炜煜(1963-),女,硕士,教授,研究方向:药物新剂型与新技术研究,E-mail:weiyuzhang2003@126.com。

中图分类号: TS207.3
计量
- 文章访问数: 234
- HTML全文浏览量: 3
- PDF下载量: 24
出版历程
- 收稿日期: 2020-04-25
- 网络出版日期: 2021-03-15
- 刊出日期: 2021-03-14

Safety Evaluation of Jingshen Tablet and Its Anti-acute Alcoholic Liver Injury

1. School of Pharmaceutical Sciences Changchun Universiry of Chinese Medicine, Changchun 130117, China;
2. Department of The First Hospital of Jilin University, Changchun 130021, China

摘要

摘要: 目的：本文对景参片进行安全性评价，并研究其对急性酒精性肝损伤的辅助保护功能。方法：通过小鼠急性毒性试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验、Ames试验及大鼠30 d喂养试验，进行景参片的安全性毒理学评价。60只小鼠随机分为5组，空白对照组给予无菌水，模型对照组及各剂量组给予50%乙醇建立酒精性肝损伤模型。造模成功后，将景参片经口灌胃，按人体推荐剂量（4.8 g/60 kg·BW）的5、10、30倍（0.40、0.80、2.40 g·kg^-1）给药，记录并观察景参片对小鼠肝组织中丙二醛（MDA）、还原型谷胱甘肽（GSH）和甘油三酯（TG）含量的影响。结果：小鼠急性经口试验最大给药量均大于15 g/（kg·BW），急性毒性分级属无毒级；小鼠各剂量组骨髓嗜多染红细胞微核率、精子畸形发生率与溶剂对照组比较均无显著性差异（P>0.05），Ames试验中各剂量组回变菌落数均未超过溶剂对照组、未处理对照组回变菌落数2倍以上，说明景参片无诱发突变作用；大鼠30 d喂养试验脏器指数、生化及血液指标与溶剂对照组比较均无显著性差异（P>0.05）；与模型对照组比较，2.40 g·（kg·BW）^-1组能显著降低肝组织中丙二醛及甘油三酯含量（P<0.05）；病理结果显示，景参片各剂量组均能减轻肝细胞中的脂肪变性及炎性浸润。结论：景参片具有抗急性酒精性肝损伤作用，在受试剂量范围内，安全无毒。
- 景参片 /
- 安全性 /
- 肝损伤 /
- 保健食品
Abstract: Objective: This article evaluates the safety of Jingshen Tablets and studies its auxiliary protective function on acute alcoholic liver injury. Methods: Through the acute toxicity test in mice, the micronucleus test in mouse bone marrow polychromatic erythrocytes, the sperm deformity test in mice, the Ames test and the 30-day feeding test in rats, the safety and toxicological evaluation of Jingshen tablets were carried out.60 mice were randomly divided into 5 groups. The control group of the blank group was given sterile water, and the model control group and each dose group were given 50% ethanol to establish an alcoholic liver injury model. After successful model building, the Jingshen tablets were administered orally and administered at 5, 10, and 30 times(0.40, 0.80, 2.40 g·kg^-1) of the recommended human dose(4.8 g/60 kg·BW), and the scene was recorded and observed. The effect of ginseng tablets on the contents of malondialdehyde(MDA), reduced glutathione(GSH) and triglyceride(TG) in mouse liver tissue.Results: The maximum dose in the acute oral test in mice was all greater than 15 g/(kg·BW), and the acute toxicity was classified as non-toxic, the micronucleus rate of bone marrow polychromatic erythrocytes and the incidence of sperm abnormalities in each dose group of mice were no significant difference(P>0.05), compared with those in the solvent control group. In the Ames test, the number of reverted colonies in each dose group did not exceed the number of reverted colonies in the solvent control group and the untreated control group by more than 2 times, indicating that Jingshen tablets have no mutagenic effect, There was no significant difference in organ index, biochemical and blood indexes between rats fed for 30 days and solvent control group(P>0.05), compared with model control group, 2.40 g·(kg·BW) ^-1 group could significantly reduce liver tissue the content of malondialdehyde and triglyceride in the medium(P<0.05). The pathological results showed that all dose groups of Jingshen tablets could alleviate steatosis and inflammatory infiltration in liver cells. Conclusion: Jingshen tablets have the effect of anti-acute alcoholic liver injury, and are safe and non-toxic within the scope of the dose.
- Jingshen tablet /
- safety /
- liver injury /
- functional food

HTML全文

参考文献(25)

[1]	陈绍红,钟赣生,刘明,等.枳榧具子解酒作用的药用部位记载[J].科技导报,2013,31(Z2):107-111.
[2]	李志满,邵紫君,李珊珊,等.人参枳椇子提取物对小鼠酒精性肝损伤的保护作用[J].食品工业科技,2019,40(14):302-306 ,313.
[3]	陈春晓,朱肖鸿.酒精性肝炎大鼠模型建立及枳椇子的干预作用[J].浙江中西医结合杂志,2007,17(5):285-287.
[4]	余选良,朱肖鸿,冯舒婷.枳椇子治疗酒精性肝病现状[J].浙江中西医结合杂志,2017,27(4):342-344.
[5]	叶倩男,徐列明,平键.红景天苷保肝作用及相关机制的研究进展[J].上海中医药大学学报,2020,34(1):83-87 ,100.
[6]	Xu N,Huang F,Jian C,et al. Neuroprotective effect of salidroside against central nervous system inflammation-induced cognitive deficits:A pivotal role of sirtuin 1-dePendent Nrf-2/HO-1/NF-KB Pathway[J]. Phytother Res,2019,33(5):1438-1447.
[7]	Xu J,Li Y. Effects of salidroside on exhaustive exercise-induced oxidative stress in rats[J]. Mol Med ReP,2012,6(5):1195-1198.
[8]	陈娅,蔡静,李勇,等.海参胶原低聚肽对抗结核药物性肝损伤改善效果[J].中国食物与营养,2018,24(5):68-72.
[9]	Orman E S,Odena G,Bataller R. Alcoholic liver disease:Pathogenesis,management,and novel targets for therapy[J]. Journal of Gastroenterology and Hepatology,2013,28(1):77-84.
[10]	孙晓梅,阎姝,田书霞. 中药治疗肝损伤的研究进展[J]. 中药材,2016,39(11):2661-2664.
[11]	史保银,刘新宇,邸琳,等. 葛根护肝片组方配伍及其辅助护肝功能研究[J].食品工业科技,2020,41(10):299-305.
[12]	瞿文生,尹继业,高月求,等.天然多糖对化学性肝损伤的干预和治疗效果评价研究进展[J].国际药学研究杂志,2018,45(12):885-898.
[13]	Leung T M,Nieto N. CYP2E1 and oxidant stress in alcoholic and nonalcoholic fatty liver disease[J]. Journal of Hepatology,2013,58:395-398.
[14]	耿文学,丁宏翼,易云苏.枳椇子提取物对实验性大鼠肝纤维化的防治作用[J].中药材,2008,31(10):1550-1552.
[15]	李钇垚,蹇瑜璇,窦纯,等. 红景天苷对呋喃所致小鼠肝损伤的保护作用研究[J].农产品加工,2019(15):53-57,60.
[16]	王丹,丁琳,董平,等. 海参皂苷对脂肪肝大鼠胆固醇代谢的调节作用[J].营养学报,2016,38(1):67-70.
[17]	赵麟萱,高卓林,金向群,等.保肝软胶囊对化学性肝损伤的辅助保护作用[J].特产研究,2019,41(2):62-65.
[18]	李谚语,刘丽,范颖,等.保肝汤对小鼠四氯化碳致慢性化学性肝损伤的保护作用[J].天津中医药,2018,35(12):935-938.
[19]	Fubini B. Surface reactivity in the Pathogenic response to particulates[J].Environ Health Perspect,1997,105(15):1013-1120.
[20]	赵春红,张力,姜淑卿,等. 4种常见残留农药联合作用对小鼠遗传毒性研究[J].职业与健康,2018,34(20):2774-2781.
[21]	曾惠芬,戴卫波,欧焕娇.素馨护肝方对ANIT诱导的大鼠胆汁淤积肝损伤的保护作用[J].海南医学,2019,30(13):1633-1638.
[22]	Yao P,Nussler A,Liu L,et al. Quercetin protects human hepatocytes from ethanol-derived oxidative stress by inducing heme oxygenase-1 via the MAPK/Nrf2 Pathways[J]. Journal of HePatology,2007,47(2):253-261.
[23]	向晓燕,余忠姝,张敏,等.吴茱萸碱复合纳米粒对小鼠急性肝损伤的保护作用[J/OL].中国医院药学杂志:1-5[2020-05-29 ].http://kns.cnki.net/kcms/detail/42.1204.R.20200508.0905.014.html.
[24]	王楠,杜双奎,冯宪超,等.枳椇乳酸菌发酵饮料对小鼠酒精肝损伤的保护作用[J].现代食品科技,2016,32(8):28-33 ,41.
[25]	刘泽鑫,刘畅,钱和.芦荟多糖联合低聚果糖缓解小鼠酒精性肝损伤[J].现代食品科技,2019,35(5):68-74 ,295.