Extraction Process Optimization and Physicochemical Properties of Polysaccharide from Wheat Germ Modified by Steam Explosion
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摘要: 为有效提高小麦麦胚多糖提取得率，本文采用蒸汽爆破技术对小麦麦胚进行前处理，再结合响应面法对蒸汽爆破麦胚多糖提取工艺进行优化。结果得出最优提取工艺条件为：提取时间为30 min，提取温度为70℃，料液比为1：5（g/mL），提取次数为3次，在此条件下的多糖的平均提取得率达到18.72%。该条件下提取的多糖其重均分子量主要集中在2.26×105、5.91×105和1.76×106Da，其峰面积比分别为14.56%、17.73%和67.71%，其表面结构呈片状不规则破碎结构，且具有良好的持油性能。这些结果初步揭示了蒸汽爆破麦胚多糖的特性，为进一步研究蒸汽爆破麦胚多糖的性质提供了理论依据。Abstract: In order to improve the extraction yield of wheat germ polysaccharide,the steam explosion technology was used to pre-treat wheat germ,and then the response surface method was used to optimize the extraction conditions of wheat germ polysaccharide. The optimized extraction conditions of steam explosion wheat germ polysaccharide were the following: extraction time 30 min;extraction temperature 70 ℃;solid-liquid ration 1∶5 (g/mL)and repetition of the extracting procedure 3 times. Under such conditions,polysaccharide yield was 18.72%. The average molecular weight of polysaccharides was mainly composed of 2.26×105,5.91×105 and 1.76×106 Da,and the peak area ratio was 14.56%,17.73% and 67.71%,respectively. The polysaccharide surface structure was flaky with irregular broken,and had good oil holding ability. These results show some preliminary characteristics of the wheat germ polysaccharide,providing a theoretical basis for further understanding wheat germ polysaccharide properties.
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[1] Rizzello C G,Nionelli L,Coda R,et al. Effect of sourdough fermentation on stabilisation,and chemical and nutritional characteristics of wheat germ[J]. Food Chemistry,2010,119(3):1079-1089.
[2] Niu L Y,Jiang S T,Pan L J,et al. Characterization of wheat germ oil in terms of volatile compounds,lipid composition,thermal behavior,and structure[J]. International Journal of Food Properties,2013,16(8):1740-1749.
[3] Sun R,Zhang Z,Hu X,et al. Effect of wheat germ flour addition on wheat flour,dough and Chinese steamed bread properties[J]. Journal of Cereal Science,2015,64:153-158.
[4] Veraverbeke W S,Delcour J A. Wheat protein composition and properties of wheat glutenin in relation to breadmaking functionality[J]. Critical Reviews in Food Science and Nutrition,2002,42(3):179-208.
[5] Yun L,Li D,Yang L,et al. Hot water extraction and artificial simulated gastrointestinal digestion of wheat germ polysaccharide[J]. International Journal of Biological Macromolecules,2019,123:174-181.
[6] Ghafoor K,Özcan M M,Juhaımı F A,et al. Nutritional composition,extraction,and utilization of wheat germ oil:A review[J]. European Journal of Lipid Science and Technology,2017,119(7):1-9.
[7] Zhu K,Zhou H. Purification and characterization of a novel glycoprotein from wheat germ water-soluble extracts[J]. Process Biochemistry,2005,40(3-4):1469-1474.
[8] 赵彬,张海晖,张继贤,等. 小麦胚芽多糖的亚临界水萃取的工艺研究[J]. 食品工业,2017,38(7):45-49. [9] Wang L,Xu H,Yuan F,et al. Preparation and physicochemical properties of soluble dietary fiber from orange peel assisted by steam explosion and dilute acid soaking[J]. Food Chemistry,2015,185:90-98.
[10] Yu Z,Zhang B,Yu F,et al. A real explosion:The requirement of steam explosion pretreatment[J]. Bioresource Technology,2012,121:335-341.
[11] 原义涛,陈洪章. 蒸汽爆破技术在麻黄碱提取中的应用[J]. 中国药科大学学报,2006,36:414-416. [12] Chen G,Chen H. Extraction and deglycosylation of flavonoids from sumac fruits using steam explosion[J]. Food Chemistry,2011,126:1934-1938.
[13] Persson T,Dinh E,Jönsson A S. Improvement of arabinoxylan isolation from barley husks[J]. Food and Bioproducts Processing,2009,87(3):228-233.
[14] 谢璇. 麦麸对面团特性的影响及其作用机理[D]. 天津:天津科技大学,2017. [15] Robyt J F,Bemis S. Use of the autoanalyzer for determining the blue value of the amylose-iodine complex and total carbohydrate by phenol-sulfuric acid[J]. Analytical Biochemistry,1967,19(1):56-60.
[16] Zhu J,Chen Z,Chen L,et al. Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea[J]. International Journal of Biological Macromolecules,2019,130:388-398.
[17] 孟雅倩. 挤压处理对燕麦多糖加工特性及结构的影响[D]. 天津:天津科技大学,2016. [18] Sui W,Xiao Y,Liu R,et al. Steam explosion modification on tea waste to enhance bioactive compounds' extractability and antioxidant capacity of extracts[J]. Journal of Food Engineering,2019,261:51-59.
[19] Shevchenko S,Chang K,Robinson J,et al. Optimization of monosaccharide recovery by post-hydrolysis of the water-soluble hemicellulose component after steam explosion of softwood chips[J]. Bioresource Technology,2000,72(3):207-211.
[20] Persson T,Ren J L,Joelsson E,et al. Fractionation of wheat and barley straw to access high-molecular-mass hemicelluloses prior to ethanol production[J]. Bioresource Technology,2009,100(17):3906-3913.
[21] Chen C,You L J,Abbasi A M,et al. Optimization for ultrasound extraction of polysaccharides from mulberry fruits with antioxidant and hyperglycemic activity in vitro[J]. Carbohydrate Polymers,2015,130:122-132.
[22] Tsubaki S,Oono K,Hiraoka M,et al. Microwave-assisted hydrothermal extraction of sulfated polysaccharides from Ulva spp. and Monostroma latissimum[J]. Food Chemistry,2016,210:311-316.
[23] 白雨鑫,郭斌,韩冠英,等. 萝藦果壳多糖提取工艺优化及其抗氧化活性研究[J]. 食品工业科技,2015,36(20):278-283. [24] Sui W,Xie X,Liu R,et al. Effect of wheat bran modification by steam explosion on structural characteristics and rheological properties of wheat flour dough[J]. Food Hydrocolloids,2018,84:571-580.
[25] Yan X,Ye R,Chen Y. Blasting extrusion processing:The increase of soluble dietary fiber content and extraction of soluble-fiber polysaccharides from wheat bran[J]. Food Chemistry,2015,180:106-115.
[26] 郭婷婷,阳雁,谢笔钧,等. 米糠可溶性膳食纤维对降低蛋黄酱中胆固醇含量及流变特性的影响[J]. 食品工业科技,2015,36(20):265-270. [27] 高晓光,冯随,杨涛,等. 麦麸膳食纤维对乳化型香肠品质的影响研究[J]. 食品工业科技,2016,37(6):151-154 ,159.
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