Processing Comparative of Solvent Extraction and Ultrasonic-assisted Extraction for Polyphenols from Lycium ruthenicum Murr.
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摘要: 探讨了黑果枸杞多酚适宜的提取工艺。通过单因素实验和Box-Behnken（BB）试验设计法对黑果枸杞多酚的提取参数进行优化，对溶剂提取法与超声辅助法进行了比较。结果表明，响应面优化后溶剂法提取黑果枸杞多酚的最佳参数为：乙醇体积分数70%，提取温度58℃，提取时间37 min，液料比50：1 mL/g，多酚得率为35.9021 mg/g，与理论预测值的相对标准偏差为0.95%；超声辅助法提取黑果枸杞多酚的最佳参数为：乙醇体积分数60%，液料比50：1 mL/g，提取温度36℃，提取时间31 min，超声功率240 W，黑果枸杞多酚的得率为39.6845 mg/g，与理论预测值的相对标准偏差为0.29%。比较发现，溶剂法乙醇用量为超声辅助法的1.16倍，提取温度较超声辅助法高22℃且提取时长延长16.22%，因此，超声辅助法工艺简单、经济、省时、能耗低、提取率高，为黑果枸杞多酚的深入研究、应用开发提供了参考依据。Abstract: The suitable extraction technology of polyphenols from Lycium ruthenicum Murr. was investigated. Single factor experiments and Box-Behnken(BB)experimental design method were adopted to optimize the extraction technology parameters,and the solvent extraction and ultrasonic assisted extraction were compared in this paper. The results showed that the optimum extraction parameters of solvent extraction were:Ethanol volume fraction 70%,liquid to solid ratio 50:1 mL/g,extraction temperature 58 ℃,extraction time 37 min,and the yield of Lycium ruthenicum Murr. polyphenols was 35.9021 mg/g with the RSD to the predicted value was 0.95%. The optimum extraction parameters of ultrasonic assisted extraction were:Ethanol volume fraction 60%,liquid to solid ratio 50:1 mL/g,extraction temperature 36 ℃,extraction time 31 min and ultrasonic power 240 W,and the yield of Lycium ruthenicum Murr. polyphenols was 39.6845 mg/g with the RSD to the predicted value was 0.29%. It was found that the solvent extraction method had more ethanol dosage with 1.16 times than ultrasonic assisted extraction,the extraction temperature 22 ℃ higher and extended the extraction time 16.22% than the latter. Therefore,the ultrasonic-assisted extraction process had advantages of simple process,time-saving,low energy consumption and high efficiency,which would provide foundation for further study of Lycium ruthenicum Murr. polyphenols.
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