Abstract: In order to explore the effects of different modified atmosphere preservation methods on the fresh-keeping of Lentinus edodes,Lentinus edodes were persevered with polyethylene(PE)modified atmosphere package and high CO₂ controlled atmosphere and stored at 4±1 ℃ for 20 d. Lentinus edodes without any treatment as control. Changes of protein,reducing sugar,ascorbic acid,total phenol,flavonoid,malondialdehyde and the activity of browning related enzyme such as peroxidase(POD),polyphenol oxidase(PPO)and phenylalanine ammonia lyase(PAL)in the mushroom tissue were measured. The results showed that the indicators of the Lentinus edodes treated with two kinds of modified atmosphere preservation methods were significantly better than the control. PE modified atmosphere package treatment could significantly inhibit the activities of POD,PPO and PAL,reduce the level of MDA,the nutrition loss of protein,reducing sugar and ASA,maintain high content of total phenols and flavonoids. And then,the process of cell aging was delayed. Furthermore,the sensory score of Lentinus edodes was improved. Therefore,PE modified atmosphere package could be a better method for Lentinus edodes storage and preservation than the high CO₂ controlled atmosphere.