Effect of Exogenous CO on Proteome of Postharvest Jujube Infected by Aternaria alternate
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摘要: 为了阐明外源CO处理对链格孢菌（Aternaria alternata）浸染过程中的枣果实蛋白组影响，采用同位素标记蛋白组分析技术测定链格孢菌入侵枣果实过程中枣果实的蛋白质表达水平，鉴定差异蛋白，并对差异蛋白进行基因本体（gene oncology，GO）及基因通路（kyoto encyclopedia of genes and genomes，KEGG）富集分析。共鉴定到蛋白2158个，其中差异蛋总数为1053个，上调差异蛋白443个，下调差异蛋白610个。GO富集分析发现，差异蛋白主要定位在细胞液、细胞质、细胞膜和细胞器中；蛋白结合、脱落酸结合、类异戊二烯结合、激素结合、乙醇结合、磷酸酶抑制剂活性和单羧酸结合是差异蛋白7种主要的分子功能；单一生物代谢过程、单一生物细胞过程和单一生物过程是差异蛋白3种共有的生物过程。综合GO和KEGG分析结果表明，差异蛋白主要富集在5个功能过程，分别为碳水化合物和能量代谢、氨基酸和脂类代谢、次生代谢产物的生物合成和防御相关蛋白、信号的感知和信号转导、转录和转录后调控。以上结果为进一步探索CO在枣果实抵御采后病原菌入侵中的作用机制提供理论支持。
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关键词:
- CO /
- 枣 /
- A.alternata /
- 蛋白组学 /
- 蛋白功能
Abstract: In order to explore the effect of exogenous CO on proteome of postharvest jujube infected by Aternaria alternata,the protein expression level during the invasion of A.alternata was determined by using isobaric tag for relative and absolute quantification proteomic analysis methods in this experiment,and the differentially expressed proteins were identified,GO(gene oncology)and KEGG(kyoto encyclopedia of genes and genomes)pathway of them were analyzed. The results indicated that a total of 2158 proteins were identified,of which the total number of differentially expressed proteins was 1053,of which the number of up-regulated differentially expressed proteins was 443 and the number of down-regulated differentially expressed proteins was 610.GO enrichment analysis revealed that the differential proteins were mainly located in the cytosol,cytoplasm,cell membrane and organelles. Protein binding,abscisic acid binding,isoprenoid binding,hormone binding,ethanol binding,phosphatase inhibitor activity and monocarboxylic acid binding were the seven main molecular functions of differential proteins. Single-organism metabolic process,single-organism cellular process and single-organism process were the three shared biological processes of differential proteins.GO and KEGG pathway enrichment analysis showed that the differential proteins were mainly enriched in five main functions and processes,carbohydrate and energy metabolism,amino acid and lipid metabolism,biosynthesis of secondary metabolites and defense-related proteins,signal perception and signal transduction,transcription and post-transcriptional regulation,respectively. The above results provide theoretical support for further exploring the mechanism of CO in the protection of jujube fruit against postharvest pathogen invasion.-
Keywords:
- CO /
- jujube /
- A. alternate /
- proteomics /
- protein function
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