Improvement of Astaxanthin Lipid Emulsion on the Structure and Dysfunction of Gut Microbiota Induced by High-fat Diet
-
摘要: 本文主要研究虾青素脂乳剂（astaxanthin lipid emulsion，ASTE）对高脂饮食诱导的肠道菌群紊乱的调节作用。将32只C57/BL6J雄性小鼠随机分为正常组（N）、模型组（M）、虾青素组（MA）和虾青素脂乳剂组（MAL）。使用45%高脂饲料喂养小鼠56 d建立高脂动物模型，连续灌胃30 mg/（kg·bw）的ASTE或虾青素（AST）28 d后，取结肠内容物进行宏基因组测序，利用生物信息学分析软件分析小鼠肠道菌群。结果表明，高脂饮食导致小鼠肠道菌群α-多样性显著下降8.00%（P<0.05），ASTE和AST均可以恢复菌群多样性。另外，ASTE可以调节与降脂减肥相关的拟杆菌（Bacteroidetes）、梭菌（Clostridiales）、颤螺旋菌（Oscillospiraceae）的相对丰度，并上调甘油磷脂代谢通路，推测其可以通过影响肠道菌群结构及功能改善宿主脂质代谢。本研究为开发以ASTE为功能因子的保健食品奠定了理论基础。Abstract: This article focused on the regulation of astaxanthin lipid emulsion(ASTE)on high-fat diet-induced gut microbiota disturbance. 32 C57/BL6J male mice were randomly divided into normal group(N),model group(M),astaxanthin group(MA)and astaxanthin lipid emulsion group(MAL). The mice were fed with 45% high-fat diet for 56 d to establish a high-fat model. After continuous gavage of astaxanthin(AST)or ASTE at 30 mg/(kg·bw)for 28 d,the contents of the colon were taken and total DNA was extracted. The gut microbiota was detected and analyzed by metagenome sequencing technology and bioinformatics analysis software. The results showed that high-fat diets caused a significant decrease in the gut microbiota α-diversity in mice by 8.00%(P<0.05),and AST and ASTE could restore the diversity. In addition,ASTE could regulate the relative abundance of Bacteroidetes,Clostridiales,Oscillospiraceae related to lipid-lowering and weight-loss. It was speculated that it could improve host lipid metabolism by affecting the structure and function of gut microbiota. This research lays a theoretical foundation for the development of health foods with ASTE as the functional factor.
-
Keywords:
- astaxanthin lipid emulsion /
- astaxanthin /
- high-fat diet /
- gut microbiota /
- metagenome
-
[1] Paternoster S,Falasca M.The intricate relationship between diabetes,obesity and pancreatic cancer[J].Biochimica et Biophysica Acta(BBA)-Reviews on Cancer,2020,1873(1):188326.
[2] NCD Risk Factor Collaboration(NCD-RisC).Trends in adult body-mass index in 200 countries from 1975 to 2014:A pooled analysis of 1698 population-based measurement studies with 19.2 million participants[J].The Lancet,2016,387(10026):1377-1396.
[3] 肖桂珍,易万华,苏磊.肠道菌群变化对中暑肠黏膜屏障的影响[J].解放军医学杂志,2017,42(9):838-842. [4] Dong Je,Liu J,Hu X,et al. The effect of high-fat diet on intestinal function[J]. Chemistry of Life,2019,39(6):1098-1106.
[5] Bekkering P,Jafri I,van Overveld F J,et al.The intricate association between gut microbiota and development of type 1,type 2 and type 3 diabetes[J].Expert Review of Clinical Immunology,2013,9(11):1031-1041.
[6] Hart A L,Stagg A J,Frame M,et al.The role of the gut flora in health and disease,and its modification as therapy[J]. Alimentary Pharmacology & Therapeutics,2002,16(8):1383-1393.
[7] Bruce-Keller A J,Salbaum J M,Luo M,et al.Obese-type gut microbiota induce neurobehavioral changes in the absence of obesity[J].Biological Psychiatry,2015,77(7):607-615.
[8] 孙长豹,刘志静,刘飞,等.食物成分对肠道菌群结构的影响[J].食品研究与开发,2018,39(9):178-182. [9] Miyake S,Ngugi D K,Stingl U.Diet strongly influences the gut microbiota of surgeonfishes[J].Molecular Ecology,2015,24(3):656-672.
[10] Ambati R R,Phang S M,Ravi S,et al.Astaxanthin:Sources,extraction,stability,biological activities and its commercial applications:A review[J].Marine Drugs,2014,12(1):128-152.
[11] Martin H D,Jäger C,Ruck C,et al.Anti-and prooxidant properties of carotenoids[J].Journal Für Praktische Chemie,1999,341(3):302-308.
[12] Boussiba S.Carotenogenesis in the green alga Haematococcus pluvialis:Cellular physiology and stress response[J].Physiologia Plantarum,2000,108(2):111-117.
[13] Wang J H,Liu S W,Wang H,et al.Xanthophyllomyces dendrorhous-derived astaxanthin regulates lipid metabolism and gut microbiota in obese mice induced by a high-fat diet[J]. Marine Drugs,2019,17(6):337.
[14] Liu H L,Liu M H,Fu X Q,et al.Astaxanthin prevents alcoholic fatty liver disease by modulating mouse gut microbiota[J].Nutrients,2018,10(9):E1298.
[15] Soukoulis C,Bohn T.A comprehensive overview on the micro-and nano-technological encapsulation advances for enhancing the chemical stability and bioavailability of carotenoids[J]. Critical Reviews in Food Science and Nutrition,2018,58(1):1-36.
[16] 姜燕蓉,刘锴锴,齐筱莹,等.虾青素的生物功效及其运载体系研究现状[J].食品与发酵工业,2019,45(13):250-256. [17] Anarjan N,Jafarizadeh-Malmiri H,Nehdi I A,et al.Effects of homogenization process parameters on physicochemical properties of astaxanthin nanodispersions prepared using a solvent-diffusion technique[J].International Journal of Nanomedicine,2015,10:1109-1118.
[18] Zhou Q X,Xu J,Yang L,et al.Thermal stability and oral absorbability of astaxanthin esters from Haematococcus pluvialis in Balb/c mice[J].Journal of the Science of Food and Agriculture,2019,99(7):3662-3671.
[19] 高远,杨鲁,袁诗涵,等.虾青素酯对高脂高糖饮食致小鼠胰岛素抵抗的影响[J].食品工业科技,2019,40(23):290-295. [20] Shao T Y,Yu Q H,Zhu T S,et al.Inulin from Jerusalem artichoke tubers alleviates hyperglycaemia in high-fat-diet-induced diabetes mice through the intestinal microflora improvement[J].British Journal of Nutrition,2020,123(3):308-318.
[21] Ren D Y,Gong S J,Shu J Y,et al.Effects of mixed lactic acid bacteria on intestinal microbiota of mice infected with Staphylococcus aureus[J].BMC Microbiology,2018,18(1):109.
[22] Arif S,Reitner J,Hoppert M. Composition,diversity and functional analysis of the modern microbiome of the middle triassic cava superiore beds(Monte San Giorgio,Switzerland)[J].Scientific Reports,2019,9:20394.
[23] 冯涵.紫苏油与虾青素联合作用调节高脂小鼠脂代谢与肠道菌群的研究[D].武汉:武汉轻工大学,2018. [24] Shen L D,Wu H S,Gao Z Q,et al.Comparison of community structures of Candidatus Methylomirabilis oxyfera-like bacteria of NC10 Phylum in different freshwater habitats[J].Scientific Reports,2016,6:25647.
[25] Zhang J Y,Chen M X,Sui Q W,et al.Impacts of addition of natural zeolite or a nitrification inhibitor on antibiotic resistance genes during sludge composting[J].Water Research,2016,91:339-349.
[26] Glasl B,Herndl G J,Frade P R.The microbiome of coral surface mucus has a key role in mediating holobiont health and survival upon disturbance[J].The ISME Journal,2016,10(9):2280-2292.
[27] Wang K,Liao M F,Zhou N,et al.Parabacteroides distasonis alleviates obesity and metabolic dysfunctions via production of succinate and secondary bile acids[J].Cell Reports,2019,26(1):222-235.
[28] Jennings A,Koch M,Jensen M K,et al.The role of the gut microbiome in the association between habitual anthocyanin intake and visceral abdominal fat in population-level analysis[J].The American Journal of Clinical Nutrition,2020,111(2):340-350.
[29] del Chierico F,Abbatini F,Russo A,et al.Gut microbiota markers in obese adolescent and adult patients:Age-dependent differential patterns[J].Frontiers in Microbiology,2018,9:1210.
[30] 靖丽.柑橘精油的代谢图谱及其主要成分d-柠檬烯对糖脂代谢紊乱的防治作用研究[D].重庆:西南大学,2014. -
期刊类型引用(5)
1. 王共明，黄会，丁玉竹，薛敬林，舒志强，井月欣，矫春娜，张健. 海参粉超临界CO_2萃取脱脂工艺优化及对挥发性风味物质的影响. 食品工业科技. 2025(03): 241-248 . 
本站查看
2. 李正阳，刘畅，费靖淳，周浩，韩万鑫，潘一萍，祁艳霞，赵前程. 刺参自溶肽美拉德反应产物表征及抗氧化活性研究. 食品安全质量检测学报. 2025(04): 224-233 . 百度学术
3. 赵影，田柬昕，钟碧銮，李萌，苏可珍. 海参深加工脱腥技术研究进展. 食品工业. 2024(03): 229-235 . 百度学术
4. 王志龙，王禹，段静瑶，苏岩峰，喻佩. 海参制品腥味化合物形成与脱腥技术研究进展. 中国调味品. 2024(06): 206-212 . 百度学术
5. 马慧，顾雪敏，李芳，梅洁，王梓棚，孔令明. 菌酶协同处理在食品加工中的研究进展及应用. 中国调味品. 2024(07): 208-213 . 百度学术
其他类型引用(1)
计量
- 文章访问数: 365
- HTML全文浏览量: 23
- PDF下载量: 46
- 被引次数: 6
下载:
