蛹药菌质抗氧化活性与活性成分的量效关系分析

许彬; 周永康; 李慧星; 王华龙; 冯力康

doi:10.13386/j.issn1002-0306.2020030414

蛹药菌质抗氧化活性与活性成分的量效关系分析

1. 南阳理工学院生物与化学工程学院, 河南南阳 473004;
2. 河南省工业微生物资源与发酵重点实验室, 河南南阳 473004

基金项目:

河南省科技发展计划项目（172102110109）；河南省科技发展计划项目（162102110109）；河南省自然科学基金项目（182300410151）；南阳市科技发展计划项目（KJGG086）。

详细信息

作者简介:
许彬(1980-),女,硕士,副教授,研究方向:食品发酵技术,E-mail:nibux@163.com。

中图分类号: TS201.3
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出版历程
- 收稿日期: 2020-03-31
- 网络出版日期: 2021-01-20
- 刊出日期: 2021-01-14

Analysis of Relationship between Active Ingredient Content and Antioxidant Activity of Cordyceps militaris-Chinese Yam Fungi Substance

1. College of Biology and Chemistry Engineering, Nanyang Institute of Technology, Nanyang 473004, China;
2. Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, Nanyang 473004, China

摘要

摘要: 研究蛹药菌质抗氧化活性与活性成分含量的量效关系。利用偏最小二乘回归法建立菌质中虫草素、总酚、总黄酮、总皂苷和总多糖含量与自由基清除率的回归方程，分析蛹药菌质中抗氧化活性成分对抗氧化活性的影响。结果表明：模型中主成分个数取2时，对各个因变量的累计贡献率基本达到了85%，模型的拟合效果良好。模型中虫草素对各种自由基清除率的影响均不显著（P>0.05）；总酚含量对各个自由基清除率指标产生显著正向影响（P<0.05）；总黄酮对超氧自由基清除率产生极显著的负向影响（P<0.01），对其他自由基清除率影响不显著（P>0.05）；总皂苷对各种自由基清除率均产生正向影响，对·OH自由基清除率的影响极显著（P<0.01）；总多糖对各种自由基清除率均产生极显著的负向影响（P<0.01）。说明抗氧化活性成分含量与菌质的抗氧化活性存在量效关系，活性成分对抗氧化活性的贡献非简单加和作用且对抗氧化活性作用各不相同。
- 蛹药菌质 /
- 抗氧化活性 /
- 活性成分 /
- 量效关系
Abstract: The relationship between active ingredient content and antioxidant activity of Cordyceps militaris-Chinese Yam Fungi substance was analyzed. Partial least squares regression was used to establish regression equation of cordycepin,total polyphenols,total flavonoids,total saponins and,total polysaccharides to response of free radical scavenging rate. Then the effect of active ingredient content on antioxidant activity in Cordyceps militaris-Chinese Yam Fungi substance was analyzed. The results showed that,when the number of principal components of the model was two,85% of the total variability could be explained,and the model could fit the experimental data effectively. The influence of cordycepin on free radical scavenging rates were not significant(P>0.05). The total polyphenols had significant positive influence on free radical scavenging rates(P<0.05).The total flavonoids had very significant negative influence on superoxide free radical scavenging rate(P<0.01),but had no significant influence on other free radical scavenging rates(P>0.05). The total saponins had positive influence on free radical scavenging rate,and had very significant influence on hydroxyl free radical scavenging rates(P<0.01). The total polysaccharides had very significant negative influence on free radical scavenging rates(P<0.01). There are mathematical relationship between the active ingredient content and antioxidant activity of cordyceps militaris-Chinese Yam Fungi substance. The effect of the active ingredient on antioxidant activity are not simple superposition,and active ingredients have different effect on antioxidant activity.
- Cordyceps militaris-Chinese Yam Fungi substance /
- antioxidant activity /
- active ingredient /
- content-response relationship

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参考文献(28)

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