石墨烯修饰酶脂质体生物传感器检测重金属的研究

关桦楠; 彭勃; 薛悦; 吴巧艳; 孙璐; 迟德富; 张娜

doi:10.13386/j.issn1002-0306.2020030299

石墨烯修饰酶脂质体生物传感器检测重金属的研究

关桦楠^1, ,,
彭勃¹,
薛悦¹,
吴巧艳¹,
孙璐²,
迟德富²,
张娜¹

1. 哈尔滨商业大学食品工程学院, 黑龙江哈尔滨 150076;
2. 东北林业大学林学院, 黑龙江哈尔滨 150040

基金项目:

国家自然科学基金资助项目（31871747，31201376和31370649）；黑龙江省自然科学基金项目（C2016034）；黑龙江省普通本科高等学校青年创新人新才培养计划（UNPYSCT-2016060）；中国博士后基金资助项目（2014T70304和2013M531009）；黑龙江省博士后科研启动项目（LBH-Q19027）；哈尔滨商业大学青年创新人才支持计划（2019CX05）资助；哈尔滨商业大学大学生创新创业训练计划项目（201810240080）。

详细信息

作者简介:
关桦楠(1983-),男,博士,副教授,研究方向:食品安全快速检测分析,E-mail:guanhuanan3@163.com。

通讯作者:
关桦楠(1983-),男,博士,副教授,研究方向:食品安全快速检测分析,E-mail:guanhuanan3@163.com。

中图分类号: TS207.3
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出版历程
- 收稿日期: 2020-03-23
- 网络出版日期: 2021-03-15
- 刊出日期: 2021-03-14

Detection of Heavy Metals by Graphene-modified Enzyme Liposome Biosensor

1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China;
2. College of Forestry, Northeast Forestry University, Harbin 150040, China

摘要

摘要: 通过自组装技术将葡萄糖氧化酶脂质体与纳米石墨烯共修饰于玻碳电极表面，制备出新型葡萄糖氧化酶电化学生物传感器，并利用其构建重金属残留检测体系。结果表明，经超声处理的石墨烯片层较薄，厚度在5 nm左右；以Hg²⁺和Cu²⁺作为重金属模型，分别在0.15和0.20 V时出现氧化峰电流，且不同浓度下峰电流未发生偏移；Hg²⁺在10^-10~10^-5和10^-5~10^-2 mmol/L浓度范围内，工作曲线方程分别为I （%）=0.0244 lgC+0.3543和I （%）=0.1552 lgC+1.0219，决定系数R²分别为0.9747和0.9937，检测限分别为10.97和3.60 ng/mL；Cu²⁺在10^-10~10^-2 mmol/L浓度范围内，工作曲线方程为I （%）=0.0406 lgC+0.8582，相关系数R²为0.9885，检出限为87.70 ng/mL，说明该检测方法灵敏度高。回收率试验结果显示所建立的检测方法对检测重金属具有较高的准确度，且精密度高，具有很好的应用前景。
- 重金属离子 /
- 石墨烯 /
- 脂质体 /
- 生物传感器 /
- 食品安全
Abstract: A novel glucose oxidase electrochemical biosensor was prepared by co-modifying glucose oxidase liposomes and nanographene on the surface of a glassy carbon electrode through self-assembly technology, and using it to construct a heavy metal residue detection system. The results showed that the ultrasonically processed graphene sheets were thinner and had a thickness of about 5 nm. Hg²⁺ and Cu²⁺ were used as models respectively. Hg²⁺ and Cu²⁺ showed oxidation peak currents at 0.15 and 0.20 V, respectively, and the peak current does not shift at different concentrations. In a certain linear range, the inhibition rate had a good linear relationship with ion concentration: Hg²⁺ was in the range of 10^-10~10^-5 and 10^-5~10^-2 mmol/L, the equations were I%=(0.0244 lgC+0.3543)% and I%=(0.1552 lgC+1.0219)%, the coefficient of determination R² value were 0.9747 and 0.9937, and the detection limits were 10.97 ng/mL and 3.60 ng/mL, respectively;the fitting equation for Cu²⁺ at 10^-10~10^-2 mmol/L was I%=(0.0406 lgC+0.8582)%, the coefficient of determination R² value was 0.9885 and the detection limit was 87.70 ng/mL, which indicated that the detection method had high sensitivity. The recovery test results shows that the established detection method has high accuracy and high precision for the detection of heavy metals, and has a good application prospect.
- heavy metal ion /
- graphene /
- liposome /
- biosensor /
- food safety

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