摘要:
研究碱性蛋白酶对富硒平菇蛋白的水解作用,并优化其工艺条件。以恩施产地的富硒平菇粉为原料,选取碱性蛋白酶进行试验,研究料液比、加酶量、温度、pH、水解时间五个因素对碱性蛋白酶水解富硒平菇蛋白的影响。以水解度和蛋白质溶出率为评价指标,在单因素试验的基础上进行正交试验,优化碱性蛋白酶水解富硒平菇的工艺条件。结果表明,酶法水解富硒平菇蛋白的最佳工艺条件为:料液比1:30,碱性蛋白酶添加量为4200 U/g,水解温度55 ℃,pH为10.5,水解反应时间为4 h。在此条件下,富硒平菇蛋白水解度可达到28.46%,蛋白质溶出率为82.85%,水解所得蛋白肽中硒含量为2739.78 μg/g。本研究确定了碱性蛋白酶水解富硒平菇蛋白工艺的最佳条件。
Abstract:
To study the degradation effect of alkaline protease on selenium-rich Pleurotus ostreatus and optimize its technological conditions.Using selenium-enriched Pleurotus ostreatus powder from Enshi as raw material,alkaline protease was selected to study the effects of ratio of material to liquid,amount of enzyme,temperature,pH and hydrolysis time on the hydrolysis of selenium-enriched Pleurotus ostreatus protein by alkaline protease.Taking the degree of hydrolysis and the dissolution rate of protein as evaluation indexes,the orthogonal experiment was carried out on the basis of single factor experiment to optimize the technological conditions of hydrolysis of selenium-rich Pleurotus ostreatus by alkaline protease. The results showed that the optimum conditions of enzymatic hydrolysis of selenium-rich Pleurotus ostreatus protein were as follows:The ratio of material to liquid was 1:30,the amount of alkaline protease was 4200 U/g,the hydrolysis temperature was 55 ℃,and the pH10.5,reaction time was 4 h.Under these conditions,the degree of hydrolysis of selenium-enriched Pleurotus ostreatus protein can reach 28.46%,the dissolution rate of protein was 82.85%,and the content of selenium in the hydrolyzed protein peptide was 2739.78 μg/g.In this study,the optimum conditions for the hydrolysis of selenium-enriched Pleurotus ostreatus protein by alkaline protease were determined,which has a certain reference value for the further study of selenium-enriched Pleurotus ostreatus.
百度学术
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