Stability of Soymilk System by Biological Dissociation
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摘要: 本文研究了碱性蛋白酶生物解离0、10、20和30 min条件下豆乳体系的粒径分布、Zeta电位、流变学特性、乳化活性及乳化稳定性的变化。并通过多重光散射稳定性分析考察豆乳体系的稳定性。研究结果表明,当生物解离20 min时,豆乳体系的平均粒径为456 nm,豆乳的粒径主要分布在100~1000 nm,随着生物解离时间的增加,粒径分布更集中,平均粒径不断降低。当生物解离作用时间为30 min时,电位绝对值最大为30.3 mV。但随着解离时间的增加,乳化活性及乳化稳定性在一定程度上降低。另外,生物解离不会改变豆乳的流体性质。多重光散射分析表明,豆浆体系的粒径更加均匀和稳定。综上,生物解离技术可作为提高豆乳体系稳定性的一种手段。Abstract: In the study,the changes of particle size distribution,zeta potential,rheological properties,emulsifying activity and emulsion stability of soymilk system under alkaline protease biological dissociation at 0,10,20 and 30 min were studied. The stability of the soymilk system was investigated by multiple light scattering stability analysis. The research results showed that when the biological dissociation was 20 minutes,the average particle size of the soymilk system was 456 nm,and the particle size of the soymilk was mainly distributed in 100~1000 nm. With the increase of the biological dissociation time,the particle size distribution was more concentrated and the averoge particle size decreased continuously. When the biological dissociation time was 30 minutes,the maximum absolute value of the potential was 30.3 mV. As the dissociation time increased,the emulsification activity and emulsification stability decreased to certain extent. Biological dissociation did not change the fluid properties of soymilk. Multiple light scattering analysis showed that the particle size of the soymilk system was more uniform and stable. In conclusion,biological dissociation technology can be used as a means to improve the stability of the soymilk system.
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Keywords:
- biological dissociation /
- soymilk /
- stability /
- alkaline protease
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[1] 张根生,杨慧铎,岳晓霞,等.水酶法提取南瓜籽油过程中乳状液酶法联合化学法破乳工艺研究[J].食品与机械,2018,34(10):139-144. [2] 吴海波,覃媚,林大成,等.水酶法提取大豆油工艺中乳状液的酶法破乳研究[J].食品工业科技,2019,40(3):54-59. [3] David González-Gómez,María Concepción Ayuso-Yuste,Carmen Blanco-Roque,et al. Optimization of enzyme-assisted aqueous method for the extraction of oil from walnuts using response surface methodology[J]. Journal of Food Processing and Preservation,2019,43(11):356-366.
[4] 王欢,佟晓红,钟明明,等.微波干燥对生物解离富肽豆粉蛋白亚基及功能性的影响[J].农业机械学报,2018,49(7):363-368. [5] 钟明明,齐宝坤,孙禹凡,等.生物解离大豆膳食纤维对饼干质构及消化特性的影响[J].食品科学,2019,40(2):18-24. [6] 江连洲,佟晓红,刘宝华,等.酶种类对生物解离大豆蛋白酶解物功能性和苦味的影响[J].农业机械学报,2018,49(8):368-374. [7] 姚盛宇.多重光散射技术研究乳液体系的稳定性和流变性[D]. 南宁:广西大学,2016. [8] Shao Y,Tang C H. Characteristics and oxidative stability of soy protein-stabilized oil-in-water emulsions:Influence of ionic strength and heat pretreatment[J]. Food Hydrocolloids,2014,37(2):149-158.
[9] Shengnan W,Lingling Z,Qinghua L,et al. Rheological properties and chain conformation of soy hull water-soluble polysaccharide fractions obtained by gradient alcohol precipitation[J].Food Hydrocolloids,2019,91:34-39.
[10] Shao B Z,Qi Y L. Characterizing the structural and surface properties of proteins isolated before and after enzymatic demulsification of the aqueous extract emulsion of peanut seeds[J]. Food Hydrocolloids,2015,47(ISSN):51-60.
[11] 杭锋,艾连中,郭本恒,等. 多重光散射技术在乳体系稳定性分析中应用[J]. 中国乳品工业,2012,40(10):36-41. [12] 张兴,杨玉玲,马云,等. pH对肌原纤维蛋白及其热诱导凝胶非共价键作用力与结构的影响[J]. 中国农业学,2017,50(3):564-573. [13] 王立敏,陈思,丁俭,等. 生物解离大豆乳状液中蛋白质结构特征分析[J]. 食品科学,2018,39(14):9-15. [14] Yusoff M M,Gordon M H,Niranjan K. AqueD Aqueous extraction process of soybeans in corn fermentation[J]. John Wiley & Sons Inc,2019,96(9):441-451.
[15] 陈林,吴克刚,柴向华,等. 微射流均质预处理提高大豆分离蛋白酶解效率及酶解产物乳化性能[J]. 农业工程学报,2015,31(5):331-338. [16] 毕爽,李杨,毛惠婷,等.超声波处理蛋白质-磷脂复合物结构与功能性构效关系解析[J].食品与发酵工业,2016,42(10):61-67. [17] 毕爽,江连洲,毛惠婷,等. 超声波处理对大豆分离蛋白-磷脂相互作用及其复合物功能性质的影响[J].食品科学,2016,37(17):1-6. [18] 赵颖颖,李可,王鹏,等. 超声波处理对酪蛋白酸钠-大豆油预乳化液乳化稳定性的影响[J]. 食品科学,2017,38(3):75-80. [19] Zhao Q,Xiong H,Selomulya C,et al. Enzymatic hydrolysis of rice dreg protein:Effects of enzyme type on the functional properties and antioxidant activities of recovered proteins[J]. Food Chemistry,2012,134(3):1360-1367.
[20] Lam R S H,Nickerson M T. Food proteins:A review on their emulsifying properties using a structure-function approach[J]. Food Chemistry,2013,141:975-984.
[21] Cheng Y,Xiong Y L,Chen J. Antioxidant and emulsifying properties of potato protein hydrolysate in soybean oil-in-water emulsions[J]. Food Chemistry,2010,120(1):101-108.
[22] 孙禹凡,齐宝坤,廖一,等.微波辅助生物解离法提取大豆油工艺及作用机理研究[J].中国油脂,2019,44(7):16-22. [23] 徐莹,陈海英,周星,等.转谷氨酰胺酶交联木瓜蛋白酶水解产物改善大豆分离蛋白乳化特性的研究[J].食品工业科技,2013,34(6):113-117. [24] 朱建宇,齐宝坤,李杨,等.不同壁材对大豆生物解离乳状液微胶囊品质的影响[J].食品工业科技,2019,40(22):56-61. [25] 关天琪,刘颖,窦博鑫,等. 响应面优化转谷氨酰胺酶改善大豆分离蛋白酶解液乳化稳定性的研究[J]. 食品工业科技,2015,36(10):192-197.
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