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中国精品科技期刊2020
蒋永波, 汪开拓, 代领军, 邱玲岚, 张帮奎, 黎春红, 雷长毅, 龚月, 皮裕琳. 不同澄清工艺对柠檬果汁品质影响及柠檬苦素贮存期间降解速率预测模型建立[J]. 食品工业科技, 2020, 41(22): 35-42. DOI: 10.13386/j.issn1002-0306.2020020249
引用本文: 蒋永波, 汪开拓, 代领军, 邱玲岚, 张帮奎, 黎春红, 雷长毅, 龚月, 皮裕琳. 不同澄清工艺对柠檬果汁品质影响及柠檬苦素贮存期间降解速率预测模型建立[J]. 食品工业科技, 2020, 41(22): 35-42. DOI: 10.13386/j.issn1002-0306.2020020249
JIANG Yong-bo, WANG Kai-tuo, DAI Ling-jun, QIU Ling-lan, ZHANG Bang-kui, LI Chun-hong, LEI Chang-yi, GONG Yue, PI Yu-lin. Effects of Different Clarification Processes on Qualities in Lemon Juice and Establishment of Prediction Model on Degradation of Limonin during the Storage[J]. Science and Technology of Food Industry, 2020, 41(22): 35-42. DOI: 10.13386/j.issn1002-0306.2020020249
Citation: JIANG Yong-bo, WANG Kai-tuo, DAI Ling-jun, QIU Ling-lan, ZHANG Bang-kui, LI Chun-hong, LEI Chang-yi, GONG Yue, PI Yu-lin. Effects of Different Clarification Processes on Qualities in Lemon Juice and Establishment of Prediction Model on Degradation of Limonin during the Storage[J]. Science and Technology of Food Industry, 2020, 41(22): 35-42. DOI: 10.13386/j.issn1002-0306.2020020249

不同澄清工艺对柠檬果汁品质影响及柠檬苦素贮存期间降解速率预测模型建立

Effects of Different Clarification Processes on Qualities in Lemon Juice and Establishment of Prediction Model on Degradation of Limonin during the Storage

  • 摘要: 为优化柠檬NFC果汁的加工工艺,本研究比较了琼脂絮凝法、酶解-吸附联用法、酶解-絮凝联用法和冻融细胞破碎法对柠檬原果汁的澄清效果,并建立了柠檬原果汁及经不同澄清工艺处理获得的柠檬清汁贮存期间柠檬苦素降解模型。结果显示,四种澄清工艺均可明显提高柠檬果汁的透光度并维持品质;酶解-吸附联用法对柠檬原果汁的澄清效果要优于其它三种方法,但显著降低了清汁中总酚和抗坏血酸含量(P<0.05),该法最优参数为果胶酶添加量0.3%,纤维素酶添加量0.15%,酶解时间2 h,活性白土添加量为4%;经澄清处理的柠檬清汁样品在贮存期间柠檬苦素含量降解的速率常数(k)与Arrhenius数学模型的相关性高,且模型为一级反应动力学方程。经酶解-吸附联用法澄清处理的柠檬清汁中柠檬苦素降解反应活化能(Ea)显著(P<0.05)低于其余三种澄清处理法,说明该体系下柠檬苦素更易发生降解。因此,琼脂絮凝法、酶解-吸附联用法、酶解-絮凝联用法和冻融细胞法均可用于柠檬NFC原果汁的澄清,但在实际果汁生产中应考虑使用对功能性物质破坏小的方法。

     

    Abstract: To optimize the process of lemon NFC juice, the present study was conducted to compare the effects of the four clarification processes including agar flocculation, combination of enzymolysis and adsorption, combination of enzymolysis and flocculation and freeze-thaw technique on the clarification of lemon juice, and further establish the degradation model of limonin in the lemon raw juice and the lemon clear juice obtained by different clarification processes during storage. The results showed that, the four clarification processes could significantly promote the transmittance and maintain the quality in lemon raw juice. The combination of enzymolysis and adsorption had a more significant effect on clarification efficiency than the other three methods, but it significantly reduced the contents of total phenolics and ascorbic acid in the lemon clear juice. The optimum parameters for the clarification of the lemon raw juice by the combination of enzymolysis and adsorption were obtained by the addition with 0.3% of pectinase, 0.15% of cellulose and 4% of active clay and under the optimum conditions of enzymolysis time 2 h. The degradation rate constants of limonin contents in the samples of lemon clear juice clarified by any of the four processes was attributed to possess a high correlation with Arrhenius model and was modeled as a first-order reaction kinetics equation. The activation energy (Ea) of limonin degradation in the clear lemon juice clarified by combination of enzymolysis and adsorption were lower than the clarified samples by the other three methods, which indicated that limonin was more prone to degradation in this system. Therefore, all of the processes including agar flocculation, combination of enzymolysis and adsorption, combination of enzymolysis and flocculation and freeze-thaw technique could be applied for clarification of lemon NFC raw juice. However, the damage to the functional compositions should be avoid while the use of the clarification in actual juice production.

     

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