Abstract: To optimize the purification technology condition of flavonoid extract from sea-buckthorn berry through macroporous resin,the proper macroporous resin type was chosen through static adsorption-desorption experiments,and its adsorption mechanism was furthermore investigated by isothermal adsorption experiment and adsorption kinetics model,then the optimum technology was confirmed by the single-factor experiments of dynaminc adsorption-desorption,meanwhile with about the purified product,the anti-motor fatigue experiments in animal were performed. The experiment results showed that the D101 macroporous resin exhibited better performance of adsorption to flavonoid of sea-buckthorn berry and was easily eluted by ethyl alcohol. The adsorption quantity gradually increased with initial concentration increasing of sample and decreased with the temperature increasing. The adsorption curves in different temperatures accorded with the Langmuir model and the process fitted the pseudo-first-order kinetics equation. The optimum purification technology conditions were as follows:The initial concentration of sample solution was 3 mg/mL,the loading volume was 70 mL with flow rate of 1 mL/min,the pH value of sample solution was 5.0,and the ethanol concentration was 70%(v/v),the eluent volume was 160 mL with flow rate of 1 mL/min.After purification,the content of total flavonoids in product increased from 18.25% to 59.07%. Compared with the blank control group,the purified product groups at all doses significantly prolonged weight-loaded swimming time of mice(P<0.05),moreover the purified product groups at medium and high doses could obviously improved the concentration of lactic acid,hepatic glycogen,muscle glycogen and activity of lactic dehydrogenase in serum after sport(P<0.01). Therefore,the flavonoid of sea-buckthorn berry contributed to relieve fatigue in body and the study could provide reference for subsequent sea-buckthorn berry development.