Optimization of Androstenedione Production by Free Cell Method Using Response Surface Methodology
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摘要: 采用响应面法对游离细胞法生产雄烯二酮工艺进行了优化。首先利用Plackett-Burman实验设计筛选出影响雄烯二酮转化率的3个主要因素:植物甾醇浓度、羟丙基-β-环糊精浓度、菌体量。在这个基础上用最陡爬坡法来逼近最大响应值范围,然后利用响应面分析法确定这几个主要因子之间的交互作用和最佳条件。结果表明,当植物甾醇浓度为19.41 g/L,羟丙基-β-环糊精浓度为80.14 g/L,菌体浓度为110.55 g/L,雄烯二酮最大转化率可达67.25%,与响应面预测转化率相近,与优化前相比,雄烯二酮转化率提高了41.55%,雄烯二酮含量达8.68 g/L,生产强度为2.17 g/(L·d)。Abstract: The process of using free cell method produce androstenedione was optimized by response surface methodology. First,the Plackett-Burman experimental design was used to screen out three main factors affecting yield:Phytosterol concentration,hydroxypropyl-β-cyclodextrin concentration,and bacterial cell amount. On this basis,the steepest climbing path method was used to approach the region of maximum response value. Finally,the response surface analysis method was used to determine the interaction between the main factors and the optimal conditions. The results showed that when the phytosterols concentration was 19.41 g/L,the hydroxypropyl-β-cyclodextrinconcentration was 80.14 g/L,the bacterial cell amount was 110.55 g/L,the maximum theoretical yield of androstenedione was 67.25%. After three parallel experiments,the actual average yield was close to the predicted yield.At this time,the androstenedione concentration reached 8.68 g/L,and the production intensity was 2.17 g/(L·d).
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