摘要:
本文建立了高效液相色谱-串联质谱法快速检测芒果中链孢酚单甲醚、交链孢霉烯、细交链孢菌酮酸、格链孢酚、腾毒素和细格菌素6种链格孢霉毒素的方法。样品经改进的QuEChERS方法进一步完成萃取净化,以带皮全果和无皮果肉芒果样品为基质,在样品中加入适量的水,以1.5%甲酸-乙腈溶液为提取剂,无水MgSO4为除水剂,NaCl盐析,振荡混匀,在9500 r/min下离心5 min,取上清液加水稀释过膜,以Waters ACQUITYTM UPLC BEH C18柱(1.7 μm,2.1 mm×100 mm)分离,电喷雾正离子多反应模式监测。在本方法条件下,6种链格孢霉毒素在0.5~200 ng/mL的浓度范围内线性关系良好,R2>0.9925,在S/N=3时,检出限在0.6~3.0 μg/kg之间。在5、10、20 μg/kg三个加标水平下,6种链格孢霉毒素的回收率在82.5%~110.6%,相对标准偏差小于10%。该方法简单高效,可以用于芒果中6种链格孢霉毒素的测定。
Abstract:
A rapid method to detect six kinds of Alternaria toxins(including alternariol mono-methyl ether,altenuene,tenuazonic acid,alternariol,tentoxin and altenusin)in mango by ultra-high performance liquid chromatography-mass spectrometry was established. Further samples were extracted and purificated by improved QuEChERS method. The mango samples with peel and without peel were used as the matrix. A proper amount of water was added to the mango samples.1.5% formic acid acetonitrile solution as the extraction agent,anhydrous MgSO4 as the dehydrating agent,NaCl as salting out,which were shaked,mixed and centrifuged at 9500 r/min for 5 min. Finally the supernatant was diluted with water and separated by Waters ACQUITYTM UPLC BEH C18 column(1.7 μm,2.1 mm×100 mm)and monitored by electrospray ionization positive ion multi reaction mode. Under these conditions,the linear relationship of six kinds of Alternaria toxins was good in the concentration range of 0.5~200 ng/mL,R2>0.9925.The detection limit was 0.6~3.0 μg/kg when S/N was 3.The recoveries of six Alternaria toxins were 82.5%~110.6% and the relative standard deviations was less than 10% at 5,10,20 μg/kg. This method is simple and efficient,which could be used to detect six Alternaria toxins in mango.
百度学术
下载:
