Optimization of Melamine Detection Conditions by Nano-gold Modified Electrode
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摘要: 为研制一种新型简单低成本的电化学传感方法,用于检测食品中的三聚氰胺。采用混合自组装的方式,将甲烷氧化菌素(Mb)功能化纳米金(AuNPs)组装到修饰电极表面,制备出Mb-AuNPs修饰电极用来检测三聚氰胺。通过电化学循环伏安法研究了三聚氰胺在此修饰电极上的电化学行为。结果表明,优化后的检测条件为:PBS溶液浓度为0.1 mol/L,PBS溶液pH为6.0。在三聚氰胺体系中的检测可知,三聚氰胺浓度与峰电流呈良好的线性关系,相关系数r为0.9909,该方法检出限(LOD)为0.384×10-8 mol/L。该电极选择性和重现性好,具有实际应用价值。Abstract: In order to develop a new simple and low-cost electrochemical sensing method for detecting melamine in food. The methanobactin(Mb) functionalized gold(AuNPs) nanoparticles were assembled on the surface of the modified electrode by means of hybrid self-assembly,and the modified electrode of Mb-AuNPs was prepared for the detection of melamine. The results showed that the electrochemical behavior of melamine on this modified electrode was determined by electrochemical cyclic voltammetry. The optimized conditions were as follows:The concentration of PBS solution 0.1 mol/L,and the pH value of PBS solution 6.0. The detection in the melamine system showed that the melamine concentration had a good linear relationship with the peak current. The correlation coefficient was 0.9909. The detection limit(LOD) of the method was 0.384×10-8 mol/L. The electrode would have good selectivity and reproducibility,and has practical application value.
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Keywords:
- methanobactin /
- gold nanoparticles /
- electrode /
- melamine
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