摘要:
德沃斯氏菌(Devosia sp.)是一种可以高效脱除谷物及饲料中呕吐毒素的新型微生物菌株,其转化产物是目前研究较为安全的呕吐毒素转化产物。本研究以碳、氮源单因素实验为基础,选用8个因素进行部分因子试验(FFD),筛选出酵母浸粉、酵母膏和蔗糖3个最显著影响因素;对3个最显著影响因素进行最陡爬坡实验获得其最优点区域;再通过中心组合实验设计(CCD)进行响应面分析得到了Devosia sp.的最适发酵培养基组成为:酵母浸粉10.00 g/L,酵母膏6.49 g/L,蔗糖8.40 g/L,硫酸铵2 g/L,磷酸二氢钾2 g/L,硫酸镁0.7 g/L,氯化钙0.02 g/L和硫酸亚铁0.02 g/L。使用该培养基进行发酵验证试验,培养40 h的OD600达到21.66,与预测值(21.62)基本相符。综上,该回归模型对Devosia sp.发酵培养基的优化是可行的,且优化后较原始培养基生物量(OD600)提高了89.1%,降低了生产成本,为呕吐毒素脱毒菌株的工业生产奠定了基础。
Abstract:
Devosia sp. is a new microbial strain which can effectively remove deoxynivalenol(DON) from grain and feed. Its transformation product is a safe transformation product of deoxynivalenol. Based on the single factor experiments of carbon and nitrogen sources,eight factors including yeast extract powder,yeast extract,sucrose,potassium dihydrogen phosphate,ammonium sulfate,magnesium sulfate,calcium chloride,manganese sulfate and ferrous sulfate were selected to carry out partial factor experiment(FFD). Three most significant factors including yeast extract powder,yeast extract and sucrose were selected for steepest climbing experiment and response surface experiment. The optimum composition of fermentation medium was yeast extract powder 10.00 g/L,yeast extract 6.49 g/L,sucrose 8.40 g/L,ammonium sulfate 2 g/L,potassium dihydrogen phosphate 2 g/L,magnesium sulfate 0.7 g/L,calcium chloride 0.02 g/L and ferrous sulfate 0.02 g/L. Using this medium for fermentation verification test,OD600 reached 21.66 in 40 hours,which basically accorded with the predicted value(21.62). In summary,the regression model was feasible to optimize the fermentation medium of Devosia sp.,and the biomass of the optimized medium increased by 89.1% compared with the original medium. Meanwhile,the cost was reduced simultaneously which laid a foundation for the industrial production of Devosia sp.
百度学术
下载:
