摘要:
本文以亨氏马尾藻为原料,采用超声波辅助热水提取制备岩藻聚糖粗品(HSP0),进而对HSP0脱蛋白得到岩藻聚糖粗多糖(HSP1),利用DEAE-52纤维素交换层析法对HSP1纯化得到F1、F2和F3组分。将HSP0、HSP1、F1、F2、F3五种组分进行化学组成分析,并对HSP0和F2进行了单糖组成分析。采用糠醛比色法研究不同浓度的HSP0对体外胆酸盐的吸附能力和比较相同浓度下五种组分对体外胆酸盐的吸附能力。结果表明:五种组分中,HSP0的多糖含量最高,达到37.87%,F3的硫酸基团含量最高,达到18.56%,F2的岩藻聚糖含量最高,达到17.63%。单糖组成分析表明F2糖链上的岩藻糖和半乳糖的百分含量分别比HSP0高16.17%和16.13%。HSP0结合胆酸盐的能力随着浓度的升高而增强,在10 mg/mL后趋近于饱和;当各组分岩藻聚糖浓度为2 mg/mL时,F2和F3结合胆酸盐的能力最好,F2吸附牛磺胆酸钠的能力最强,吸附率达到13.36%,F3吸附水合胆酸钠和甘氨胆酸钠的能力最强,吸附率达到26.35%和11.35%。
Abstract:
The fucoidan complex(HSP0)was prepared by ultrasonic assisted hot water extraction from Sargassum henslowianum,and the crude polysaccharide(HSP1)was obtained by deproteinizing HSP0. The HSP1 was purified by DEAE-52 cellulose anion exchange chromatography to obtain F1,F2 and F3.The chemical composition analysis of five fucoidans of HSP0,HSP1,F1,F2 and F3 were carried out,and the monosaccharide composition analysis of HSP0 and F2 were carried out. Furfural colorimetric method was used to study the ability of different concentrations of HSP0 to bind bile salts in vitro and to compare the ability of five fucoidans to bind bile salts in vitro. The results showed that among the five components,HSP0 had the highest polysaccharide content,reaching 37.87%,F3 had the highest sulfate group content,reaching 18.56%,and F2 had the highest fucoidan content,reaching 17.64%. Monosaccharide composition analysis showed that the percentage of fucose and galactose on the F2 sugar chain were 16.17% and 16.13% higher than HSP0,respectively. The ability of HSP0 to bind bile salt increased with increasing of concentration,and approached saturation after 10 mg/mL. When the concentration of five fucoidans were 2 mg/mL,F2 and F3 had the best ability to bind cholate. F2 had the strongest ability to adsorb sodium taurocholate,the adsorption rate reached 13.36%;F3 had the strongest ability to adsorb sodium cholate and sodium glycinate,with adsorption rates of 26.35% and 11.35%.
百度学术
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