Abstract: To observe the regulating effect of polysaccharides from mixed of Schisandra chinensis and Astragalus membranaceus(SAP)on dyslipidemia and its mechanism,a rat model of hyperlipidemia induced by feeding with high fat diet was established. Then the SAP was administered orally to the hyperlipidemic rats for 8 weeks. During this period,the body weight of rats was recorded weekly. Subsequently,the levels of triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)in serum of rats,the TG and TC contents in the liver tissues were measured. HE staining was performed for observing histopathological changes in liver. The expressions of cholesterol metabolism-related proteins ATP-binding cassette transporter A1(ABCA1),liver X receptors α(LXRα),and ATP-binding cassette G1(ABCG1)in liver tissues were detected by western blotting analysis. The results showed that SAP(100 mg/kg)intragastric administration could significantly decrease the body weight,the levels of serum TC,TG,LDL-C,and TC and TG contents in the liver tissues(P<0.05),increase the level of serum HDL-C(P<0.05). Furthermore,SAP reduced the liver lipid deposition and improved hyperlipidemia in rats. At the same time,SAP significantly increased the expression of LXRα,ABCA1 and ABCG1 proteins in the liver of hyperlipidemic rats(P<0.05). In a conclusion,SAP could regulate the blood lipid of rats with hyperlipidemia,which may be related to the promotion of liver cholesterol metabolism.