Abstract: Objective:To study the effect of total flavonoids from Bitter Leaf in hyperlipidemia model mice. Method:Kunming mice were randomly divided into 6 groups:Control group,high fat model group,simvastatin positive control group and bitter leaf total flavonoids in low,medium and high groups(10,50,100 mg/kg). The mice of control group were fed with normal diet and the mice of other groups were fed with high fat diets. After 4 weeks,the mice were decapitated after measuring the body weight,and the contents of triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C)in serum were determined. Result:Compared with the model group,the content of TG,TC and LDL-C in the serum of the mice could be reduced with the treatment of the total flavonoids of bitter leaf,and the content of HDL-C was improved. Compared with high fat model group,the TG,TC and LDL-C in the mice serum of the bitter leaf total flavonoids group(100 mg/kg)were decreased by 28.29%,17.55% and 63.32%,respectively and HDL-C was increased by 44.20%. Conclusion:The total flavonoids of the bitter leaf have a significant hypolipidemic activity.