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中国精品科技期刊2020

QuEChERS/UPLC-MS/MS法同时测定梅花鹿鹿茸中7种镇静剂

黄胜广, 王玉方, 赵卉, 毕融冰, 刘炎, 王峰

黄胜广, 王玉方, 赵卉, 毕融冰, 刘炎, 王峰. QuEChERS/UPLC-MS/MS法同时测定梅花鹿鹿茸中7种镇静剂[J]. 食品工业科技, 2020, 41(10): 265-270. DOI: 10.13386/j.issn1002-0306.2020.10.044
引用本文: 黄胜广, 王玉方, 赵卉, 毕融冰, 刘炎, 王峰. QuEChERS/UPLC-MS/MS法同时测定梅花鹿鹿茸中7种镇静剂[J]. 食品工业科技, 2020, 41(10): 265-270. DOI: 10.13386/j.issn1002-0306.2020.10.044
HUANG Sheng-guang, WANG Yu-fang, ZHAO Hui, BI Rong-bing, LIU Yan, WANG Feng. Determination of 7 Kinds of Sedatives in Antler Velvet of Cervus Nippon Temminck by QuEChERS/UPLC-MS/MS[J]. Science and Technology of Food Industry, 2020, 41(10): 265-270. DOI: 10.13386/j.issn1002-0306.2020.10.044
Citation: HUANG Sheng-guang, WANG Yu-fang, ZHAO Hui, BI Rong-bing, LIU Yan, WANG Feng. Determination of 7 Kinds of Sedatives in Antler Velvet of Cervus Nippon Temminck by QuEChERS/UPLC-MS/MS[J]. Science and Technology of Food Industry, 2020, 41(10): 265-270. DOI: 10.13386/j.issn1002-0306.2020.10.044

QuEChERS/UPLC-MS/MS法同时测定梅花鹿鹿茸中7种镇静剂

基金项目: 

特色农产品质量安全风险隐患摸底排查与关键控制点评估(GJFP201701002)。

详细信息
    作者简介:

    黄胜广(1992-)男,硕士研究生,研究方向:鹿茸等经济动物产品的质量与安全评价,E-mail:824213281@qq.com。

    通讯作者:

    王峰(1962-),男,硕士,研究员,研究方向:特种经济动植物产品检测技术与质量安全风险评估,E-mail:tcswf@126.com。

  • 中图分类号: TS207.3

Determination of 7 Kinds of Sedatives in Antler Velvet of Cervus Nippon Temminck by QuEChERS/UPLC-MS/MS

  • 摘要: 采用分散固相萃取的前处理技术,建立了一种UPLC-MS/MS(ultra performance liquid chromatography-tandem mass spectrometry)法同时测定梅花鹿鹿茸中7种镇静剂的检测方法。鹿茸样品以2%氨水乙酸乙酯为提取剂,200 mg C18为净化剂。液相色谱条件:ACQUITY BEH C18柱(2.1 mm×100 mm,1.7 μm),流动相A为0.1%甲酸乙腈,B为水,柱温35 ℃;质谱条件:正离子模式(ESI+),多反应监测(multiple reaction monitoring,MRM)模式,鹿茸中镇静剂含量采用外标法定量。结果表明,7种镇静剂在0.3~100 ng/mL内线性关系良好,决定系数R2均大于0.992,7种镇静剂的LOD(limits of detection)为0.3 μg/kg,LOQ(limit of quantitation)为1 μg/kg,平均加样回收率为82.6%~107.3%,相对标准偏差RSD(relative standard deviation)均小于5%。该方法具有操作简便,净化效果显著,灵敏度高、准确性好、检出限低等优点,可用于梅花鹿鹿茸中7种镇静剂的检测。
    Abstract: A method for the simultaneous determination of 7 sedatives in antler velvet of Cervus nippon Temminck by UPLC-MS/MS was developed by using pre-treatment technology of dispersive solid phase extraction. The antler velvet sample was treated with 2% aqueous ammonia ethyl acetate as an extractant and 200 mg C18 as a purifying agent. Liquid chromatography conditions:ACQUITY BEH C18 column(2.1 mm×100 mm,1.7 μm),mobile phase A was 0.1% formic acid acetonitrile,B was water,column temperature was 35 ℃. Mass spectrometry conditions:Positive ion mode(ESI+),multiple reactions monitoring mode. The sedative contents in antler velvet were quantified by external standard method. The linearity of the 7 sedatives in 0.3~100 ng/mL was good,the correlation coefficient R2 was greater than 0.992,and the LOD of the 7 sedatives was 0.3 μg/kg,LOQ was 1 μg/kg,the average recovery rate was 82.6%~107.3%,and the RSD was less than 5%. The method had the advantages of simple operation,remarkable purification effect,high sensitivity,good accuracy and low detection limit. It could be used for the determination of 7 sedatives in antler velvet of Cervus nippon Temminck.
  • 期刊类型引用(5)

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    2. 宫瑞泽,陆雨顺,张磊,李志满,李珊珊,孙印石. 基于中药质量标志物(Q-Marker)的鹿茸质量控制标准体系构建. 中草药. 2021(09): 2851-2862 . 百度学术
    3. 许晓辉,李剑勇,朱仁愿,潘秀丽,李晨曦,王小乔. 动物源性中药材兽药残留检测技术研究进展. 中国兽药杂志. 2021(06): 45-52 . 百度学术
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    5. 胡雪,梁建英,谢瑞龙,张志伟,刘丽君,李翠枝,吕志勇. QuEChERS法在乳制品中农药残留检测中的应用. 乳业科学与技术. 2020(06): 41-45 . 百度学术

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  • 被引次数: 5
出版历程
  • 收稿日期:  2019-08-28
  • 网络出版日期:  2020-11-12
  • 刊出日期:  2020-05-14

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