Abstract: The decolorization parameters of strawberry polysaccharides(SP)were optimized by different macroporous resins with the highest decolorization rate,retention rate and selection coefficient. The chemical composition,monosaccharide composition,molecular weight,spectral characteristics,antioxidant activity and inhibition of α-glucosidase and α-amylase of strawberry polysaccharides were studied. The results suggested that NKA-9 resin offered better efficiency than other tested resins. The optimal decoloration parameters of NKA-9 resin were obtained as follows:Sample initial concentration of 30 mg/mL,static decoloration time of 60 min,temperature of 50 ℃,pH value of 8.0,dynamic decoloration processing volume of 4.5 BV with the flow rate of 3.0 BV/h. Decoloration ratio,PSTS recovery ratio and selectivity coefficient were 91.24%±1.32%,78.86%±1.21% and 8.24%±0.21% respectively. Pigment impurities were successfully removed from SP solutions after treated by NKA-9 resin,but decoloration treatment decreased the antioxidant ability of SP. SP was an acidic heteropolysaccharide which mainly contained carbohydrates(78.23%±0.56%,w/w),uronic acids(8.56%±0.19%,w/w)and proteins(6.14%±0.63%,w/w). SP was composed of rhamnose,arabinose,galactose,glucose,fructose,galacturonic acid and glucuronic acid with mass ratio of 1.00:0.67:0.69:0.80:0.65:0.29:0.69.SP exhibited inhibitory activities against α-glucosidase in a mixed inhibition type,and against α-amylase in an uncompetitive inhibition type. The enzyme inhibitory effect was enhanced significantly by decoloration.