摘要:
考察昆仑雪菊油树脂成分分析及体内外抗氧化效果。以昆仑雪菊为原料,采用CO2超临界流体萃取昆仑雪菊油树脂,采用气相色谱-质谱联用(GC-MS)技术对雪菊油树脂脂肪酸进行成分分析,体外实验测定了昆仑雪菊油树脂对羟自由基(·OH)、二苯代苦味酰基自由基(DPPH·)的清除率和总还原能力。体内实验测定了小鼠血清、肝脏和肾脏中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量和总抗氧化(T-AOC)能力。结果表明:昆仑雪菊油树脂中检测出12种脂肪酸,有4种不饱和脂肪酸,占脂肪酸总量的53.28%,其中亚油酸相对含量达到22.81%,亚麻酸含量为10.84%,油酸含量为7.29%。体外实验表明,昆仑雪菊油树脂对·OH和DPPH·均有不同程度的清除作用,且清除作用随油树脂浓度的升高而增强,其总还原能力较弱。昆仑雪菊油树脂对·OH和对DPPH·的IC50值分别为(0.7768±0.0675)、(0.8160±0.2219) mg/mL。体内实验表明,中、高剂量组昆仑雪菊油树脂能够使小鼠血清、肝脏和肾脏中超氧化物歧化酶(SOD)活力显著增强(P<0.05),血清、肝脏中丙二醛(MDA)含量显著降低(P<0.05),肝脏肾脏中总抗氧化(T-AOC)能力显著增强(P<0.05),但是对小鼠血清总抗氧化能力及肾脏中MDA的含量影响不显著。因此,适当剂量的昆仑雪菊油树脂可以增强机体的抗氧化机能。
Abstract:
The aim of this study was to investigate the in vitro and in vivo antioxidant effects of Kunlun chrysanthemum oleoresin. Kunlun chrysanthemum oleoresin was obtained by using supercritical fluid CO2 taken Kunlun chrysanthemum as raw material. The fatty acids of Kunlun chrysanthemum oleoresin were analyzed by GC-MS. The scavenging rate on ·OH,DPPH· and total reducing ability of Kunlun chrysanthemum oleoresin were measured in vitro. The activity of superoxide dismutase(SOD),the content of malondialdehyde(MDA)and the ability of total antioxidant(T-AOC)in serum,liver and kidney of mice were measured in vivo.The results showed that 12 kinds of fatty acids were detected in the Kunlun chrysanthemum oleoresin,including 4 kinds of unsaturated fatty acids,accounting for 53.28% of the total fatty acids,among which the relative content of linoleic acid was 22.81%,linolenic acid was 10.84%,and oleic acid was 7.29%. In vitro experiments showed that Kunlun chrysanthemum oleoresin had scavenging effects on ·OH and DPPH· in varying degrees,and the scavenging effect increased with the increase of oleoresin concentration,and its total reducing ability was weak. The IC50 values of Kunlun chrysanthemum oleoresin on ·OH and DPPH· were(0.7768±0.0675)and(0.8160±0.2219) mg/mL,respectively. In vivo experiments showed that in the middle and high dose group of Kunlun chrysanthemum oleoresin,the activity of SOD in serum,liver and kidney was significantly increased(P<0.05),the content of MDA in serum and liver was significantly decreased(P<0.05),and the ability of T-AOC in liver and kidney was significantly enhanced(P<0.05). However,the effect of Kunlun chrysanthemum oleoresin on the total antioxidant capacity of serum and the content of MDA in kidney was not significant. Therefore,the appropriate dosage of Kunlun chrysanthemum oleoresin can enhance the antioxidant function of the body.
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