Effects of Insoluble Dietary Fiber from Wild Apricot Flesh on Intestinal Function and Intestinal Flora of Mice
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摘要: 研究野山杏果肉不溶性膳食纤维(insoluble dietary fiber from wild apricot,IDFWA)润肠通便作用及对肠道菌群的影响。以KM小鼠为实验对象,采用地芬诺酯建立便秘模型,以小肠推进率、首粒排便时间、首粒排蓝便时间以及6 h排便量为考察指标,考察IDFWA的润肠通便作用;连续灌胃小鼠14 d低、中、高剂量(0.6、1.2、2.4 mg/(g·d))的IDFWA,采集实验小鼠的新鲜粪便进行培养和菌群鉴定、计数,考察IDFWA对肠道菌群的影响。结果表明,IDFWA各剂量可提高小鼠小肠推进率(P<0.05)、降低首粒排便时间(P<0.05)、降低首粒排蓝便时间(P<0.01),中、高剂量可增加小鼠6 h排便总质量(P<0.05);IDFWA低剂量对大肠杆菌抑有抑制作用(P<0.01),各剂量对金黄色葡萄球菌有抑制作用,但不具有统计学意义;中、高剂量对乳酸杆菌有促进作用(P<0.05),各剂量对双歧杆菌有促进作用(P<0.05)。可见IDFWA具有改善小鼠肠道功能与调节肠道菌群的作用。Abstract: To study the effects of insoluble dietary fiber from wild apricot(IDFWA)on moistening intestines and defecating and intestinal flora,KM mice were used as experimental subjects,and the constipation model was established by using diphenoxylate. The intestinal propulsion rate,the first defecation time,the first row of blue stool time and the 6 h defecation amount were taken as indicators to investigate the laxative effect of IDFWA. IDFWA was on oral tests in mice at low,medium and high doses(0.6,1.2,2.4 mg/(g·d))for 14 d. The fresh feces of mice were collected for culturing,identificating and counting microbial to examine the effects of IDFWA on the intestinal flora. The results showed that each dose of IDFWA could increase the intestinal propulsion rate(P<0.05). It could reduce the first defecation time(P<0.05)and the first sputum discharge time(P<0.01). The medium and high doses of IDFWA could increase the total mass of defecation in rats at 6 h(P<0.05). The low dose of IDFWA could inhibit Escherichia coli(P<0.01). Staphylococcus aureus was inhibited by each dose of IDFWA without statistically significant. Medium and high doses of IDFWA could promote Lactobacillus(P<0.05),and each dose could promote Bifidobacterium(P<0.05). It indicated that IDFWA could improve intestinal function and regulate intestinal flora of mice.
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Keywords:
- wild apricot flesh /
- insoluble dietary fiber /
- improvement /
- intestinal function /
- intestinal flora
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