Abstract: To investigate the effects of chitoloigosaccharides compound solid beverage(Ke Tang Ping)on intestinal microbiota of mice with type 2 diabetes,the mice were randomly divided into seven groups,including the normal group(NFD),the model group(T2DM),the Acarbose group(Acar),chitooligosaccharides group(COS),and Ke Tang Ping low dose group(LKTP),Ke Tang Ping medium dose group(MKTP),Ke Tang Ping high dose group(HKTP),respectively. After 4 weeks of administration,the intestinal contents were taken for intestinal flora analysis. The results indicated that the effective sequence of 16S sequencing of each group ranged from 130301 to 244496 reads,and its length ranged from 315 to 478 bp,OTU range was 1367~1835,the species coverage bacteria was greater than 0.99. The Venn diagram indicated that 556 OTUs were shared by 7 groups. Among them,326(NFD group),223(T2DM group),237(Acar group),118(COS group),197(LKTP group),142(MKTP group),181(HKTP group)were unique. The PCoA plots showed good parallelism and repeatability of samples in the NFD,T2DM,Acar,COS,LKTP,MKTP,and HKTP groups. Compared with the T2DM group,the COS group had a weak treatment effect,while the Acar,LKTP,MKTP and HKTP groups had strong treatment effects. Compared with the model group(T2DM),at the phylum level,when the mice were fed Ke Tang Ping,the relative abundance of Bacteroidetes increased,and the relative abundance of Firmicutes decreased. At the family level,the relative abundance of Bacteroidales_S24-7_group and Prevotellaceae increased significantly fed with Ke Tang Ping. However,the relative abundance of Lachnospiraceae and Desulfovibrionaceae decreased significantly. In conclusion,Ke Tang Ping could change the composition and diversity of gut microbes,promote the colonization of beneficial bacteria and reduce the relative abundance of opportunistic pathogens to promote intestinal health,and assist in the treatment of diseases such as type 2 diabetes.