摘要:
建立一种毛细管电泳-色谱联用法检测运动营养品中赛拉嗪、右美托咪啶、胍那苄等3种α2-受体激动剂的分析方法。本研究结合激光诱导荧光技术,以1.0 mmol/L的4-氟-7-硝基-2,1,3-苯并呋咱为衍生剂,在pH8.9、衍生温度60℃、衍生反应30 min。所得衍生产物以乙腈-磷酸钾(10 mmol/L)(55:45,v/v)为流动相,经苯基毛细管色谱填充柱进行分离后检测。结果表明,3种α2-受体激动剂能在1.0~20.0 ng/mL浓度范围内线性良好,相关系数R2为0.995~0.999,检出限均为5.0 μg/kg,定量限均为16.0 μg/kg,加标回收率达到80.5%~93.4%。本方法前处理操作简单、灵敏,为运动营养品中α2-受体激动剂分离分析提供了新手段。
Abstract:
A capillary electrophoresis-chromatography method was developed for the determination of serazine,dexmedetomidine and guanabenz in sports nutrients. This study combines laser-induced fluorescence technology and prepared with 1.0 mmol/L 4-fluoro-7-nitro-2,1,3-benzofurazan(NBD-F)as derivative agent at pH8.9,derivation temperature 60℃,and derivatization reaction for 30 minutes. Acetonitrile-potassium phosphate(10 mmol/L)(55:45,v/v)was used as mobile phase for the separation and determination of the derivatives by phenyl capillary column. The results showed that the linearity of three α2-receptor agonists was good in the concentration range of 1.0~20.0 ng/mL. The correlation coefficient R2 was 0.995~0.999,the detection limit was 5.0 ug/kg,the quantitative limit was 16.0 ug/kg,and the recovery rate was 80.5%~93.4%. This method is simple and sensitive,and provides a new method for the separation and analysis of three α2-receptor agonists in sports nutrients.
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