Abstract: A capillary electrophoresis-chromatography method was developed for the determination of serazine,dexmedetomidine and guanabenz in sports nutrients. This study combines laser-induced fluorescence technology and prepared with 1.0 mmol/L 4-fluoro-7-nitro-2,1,3-benzofurazan(NBD-F)as derivative agent at pH8.9,derivation temperature 60℃,and derivatization reaction for 30 minutes. Acetonitrile-potassium phosphate(10 mmol/L)(55:45,v/v)was used as mobile phase for the separation and determination of the derivatives by phenyl capillary column. The results showed that the linearity of three α₂-receptor agonists was good in the concentration range of 1.0~20.0 ng/mL. The correlation coefficient R² was 0.995~0.999,the detection limit was 5.0 ug/kg,the quantitative limit was 16.0 ug/kg,and the recovery rate was 80.5%~93.4%. This method is simple and sensitive,and provides a new method for the separation and analysis of three α₂-receptor agonists in sports nutrients.