摘要:
目的:提取玫瑰红景天根茎中的挥发性物质,测试抗氧化和抗菌活性,为玫瑰红景天的综合利用提供科学依据。方法:采用水蒸气蒸馏法提取玫瑰红景天根茎中的挥发性物质,经GC-MS分离和鉴定,借助GC用系列正构烷烃对各组分进行定性,面积归一化法确定各组分的相对含量;通过H2O2/Fe2+体系考察对羟基自由基的清除作用;用微量肉汤稀释法测定最小抑菌浓度(MIC)和平板转种法测定最小杀菌浓度(MBC)确定抗菌活性。结果:从玫瑰红景天挥发性物质中鉴定出53种化合物,主要组分有1-辛醇(42.72%)、香叶醇(29.85%)、苯乙醇(9.67%)、桃金娘烯醇(2.69%)、芳樟醇(1.89%)等,玫瑰红景天挥发性物质对羟基自由基有清除作用,其半抑制浓度IC50为0.0073 mg/mL,对抗菌试验所选的7个菌株均有抑制作用,对金黄色葡萄球菌ATTCC25925的MIC值是0.12 mg/mL、MBC值是0.36 mg/mL,对大肠杆菌ATCC25922的MIC值是0.58 mg/mL、MBC值是1.90 mg/mL。结论:通过水蒸气蒸馏法提取得到的玫瑰红景天根茎挥发性物质的组分丰富多样,是一种天然的抗氧化、抗菌活性物质。
Abstract:
Objective:The volatile compound from the rhizome of Rhodiola rosea was investigated by anti-oxidation activities and antimicrobial activities to provide scientific basis for the comprehensive utilization of this natural resource. Methods:The volatile compound from the rhizome of Rhodiola rosea was extracted by steam distillation method,these components in the volatile compound were separated and structurally identified by gas chromatography-mass spectrometry(GC-MS),moreover,the relative contents of these components by the peak-area normalization method adopted in gas chromatography(GC),and all components of the volatile compound was calibrated with a standard mixture of homologous n-alkane series. The scavenging effect of the volatile compounds on ·OH produced by H2O2/Fe2+ system was investigated,the antimicrobial activities were evaluated with the minimum inhibitory concentration(MIC)by broth microdilution method and the minimum bactericidal concentration(MBC)by flat plate conversion method. Results:A total of 53 compounds were identified,the components with higher contents in the volatile compound were 1-octanol(42.72%),(Z)-3,7-dimethyl-2,6-octadien-1-ol(29.85%),phenylethyl alcohol(9.67%),(-)-myrtenol(2.69%),linalool(1.89%). The volatile compound had obvious action to anti-oxidation activities of scavenging effect for ·OH,its half maximal inhibitory concentration value(IC50)was 0.0073 mg/mL. The volatile compound showed inhibitory effect on the 7 strains tested,especially showed the best antimicrobial activities against S. aureus ATCC 25925 with MIC=0.12 mg·mL-1 and MBC=0.36 mg·mL-1,and against E. coli ATCC 25922 with MIC=0.58 mg·mL-1 and MBC=1.90 mg·mL-1. Conclusin:These volatile compounds from the rhizome of Rhodiola rosea by steam distillation method were various,which was a kind of natural anti-oxidant and antimicrobial substance.
百度学术
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