Rapid Screening of Malachite Green Residues in Aquatic Products Using DBDI-MS/MS
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摘要: 建立了采用介质阻挡放电电离质谱（DBDI-MS/MS）技术对水产品中孔雀石绿（MG）及其代谢物隐色孔雀石绿（LMG）进行快速筛查的方法。样品经提取净化后，在氦气流量为3 L/min、离子源温度为230℃的DBDI离子源条件下，正离子模式下采用多反应监测（MRM）进行质谱检测。在此条件下，MG和LMG在1~20 μg/L范围内，相关系数r值分别为0.9951、0.9939，线性关系良好，MG和LMG的检出限均为1 μg/kg，样品加标回收率为61.8%~78.4%。该方法样品前处理简便，无需液相色谱分离，耗时短，适用大批量水产品中孔雀石绿非法添加的快速筛查。Abstract: A rapid screening method using DBDI-MS/MS was established for MG and LMG residues in aquatic products. The samples were extracted and purified,and then injected under the conditions of ionization temperature of 230 ℃ and He gas flow rate of 3 L/min using DBDI in the positive ion mode. The determination was conducted by tandem mass spectrometry in positive ESI mode under multiple reaction monitoring(MRM)mode. Under the optimal conditions,the experimental data of MG and LMG showed a good linear relationship between the signal and the concentration in 1~20 μg/L,with correlation coefficient(r)of 0.9951 and 0.9939. Moreover,the recoveries of MG and LMG were between 61.8% and 78.4% at three spiked levels. This method without tedious pretreatment and chromatographic separation,was simple and rapid,can meet the requirement of rapid screening and analysis of large quantities of samples.
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