Isolation,Identification and Characterization of Filamentous Fungi with Flocculation from the Sewage
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摘要: 目的:为了阐明污泥中丝状菌的种类和特性,利用常规培养技术从山东省临淄区金岭回族镇的污水水渠中分离絮凝菌。方法:利用马铃薯葡萄糖培养基、麦芽汁琼脂培养基和查氏培养基分离出的丝状菌经絮凝率测定和多次富集筛选得到3株絮凝性能稳定且活性良好的菌株,对各菌株进行形态学鉴定,通过单因素实验和正交试验分析各因素对菌株絮凝能力的影响情况。并对其中一株絮凝率高的菌株SP进行了分子生物学鉴定及絮凝率特性分析。结果:经形态学鉴定SQ为青霉属真菌,SR为丛梗孢科某丝状菌,SP经分子生物学鉴定为草酸青霉菌(Penicillium oxalicum)。SP在麦芽汁培养基,pH 6.0,150 r/min,培养温度28 ℃,摇床培养72 h后对5 g/L高岭土悬浮液的絮凝率可达67.7%。结论:分离出的草酸青霉菌等菌株在微生物絮凝剂研究及水体治理方面具有一定的理论和实际意义。Abstract: Objective:To clarify the species and characteristics of filamentous bacteria in sludge,flocculation bacteria were separated from the sewage drain in Jinling Hui Nationality Town,Linzi District,Shandong Province,by means of conventional culture technique. Methods:Three filamentous strains with stable flocculation performance and good activity were obtained by the determination of flocculation rate and multiple enrichment screening of the filamentous bacteria separated with potato glucose medium,wort AGAR medium and chagrin medium. Morphological identification of each strain was carried out. The impacts of each factor on the flocculation ability of the strain were analyzed by single factor experiment and orthogonal test. Molecular biological identification and flocculation rate characteristic analysis were conducted on one strain SP with high flocculation rate. Results:According to the morphological identification,SQ was a penicillium fungus,SR was a filamentous fungus of moniliaceae,and SP was identified as a Penicillium oxalicum by molecular biology. The flocculation rate of SP for 5 g/L kaolin suspension could reach 67.7% after shake cultivation in malt extract medium,pH 6.0,150 r/min and at 28 ℃ for 72 h. Conclusion:The strains such as Penicillium oxalicum separated are of certain theoretical and practical significance in research on microbial flocculant and water treatment.
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Keywords:
- sewage /
- filamentous bacteria /
- separation /
- flocculation effect
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期刊类型引用(1)
1. 田瑜琳,雒晓芳,朱雅雯,赵梓彤. 微生物絮凝剂ZF-1C的絮凝效果研究. 生物化工. 2020(03): 90-94 . 百度学术 其他类型引用(1)
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