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中国精品科技期刊2020

污水中具有絮凝作用丝状菌的分离鉴定及性能研究

雒晓芳, 苏强, 陈丽华, 张巍, 于加瑞

雒晓芳, 苏强, 陈丽华, 张巍, 于加瑞. 污水中具有絮凝作用丝状菌的分离鉴定及性能研究[J]. 食品工业科技, 2019, 40(21): 104-109. DOI: 10.13386/j.issn1002-0306.2019.21.017
引用本文: 雒晓芳, 苏强, 陈丽华, 张巍, 于加瑞. 污水中具有絮凝作用丝状菌的分离鉴定及性能研究[J]. 食品工业科技, 2019, 40(21): 104-109. DOI: 10.13386/j.issn1002-0306.2019.21.017
LUO Xiao-fang, SU Qiang, CHEN Li-hua, ZHANG Wei, YU Jia-rui. Isolation,Identification and Characterization of Filamentous Fungi with Flocculation from the Sewage[J]. Science and Technology of Food Industry, 2019, 40(21): 104-109. DOI: 10.13386/j.issn1002-0306.2019.21.017
Citation: LUO Xiao-fang, SU Qiang, CHEN Li-hua, ZHANG Wei, YU Jia-rui. Isolation,Identification and Characterization of Filamentous Fungi with Flocculation from the Sewage[J]. Science and Technology of Food Industry, 2019, 40(21): 104-109. DOI: 10.13386/j.issn1002-0306.2019.21.017

污水中具有絮凝作用丝状菌的分离鉴定及性能研究

基金项目: 

西北民族大学2019年开放项目(SYSKF-2019209)。

西北民族大学基本科研业务费专项(31920170169)

甘肃省自然科学基金项目(17JR5RA287)

兰州市创新创业人才项目(2018-RC-81)

详细信息
    作者简介:

    雒晓芳(1980-),女,硕士,副教授,主要从事环境微生物学方面的研究,E-mail:57697386@qq.com。

    通讯作者:

    陈丽华(1972-),女,博士,教授,主要从事土壤有机物污染修复机理与技术研究,E-mail:673126749@qq.com。

  • 中图分类号: TS201.3

Isolation,Identification and Characterization of Filamentous Fungi with Flocculation from the Sewage

  • 摘要: 目的:为了阐明污泥中丝状菌的种类和特性,利用常规培养技术从山东省临淄区金岭回族镇的污水水渠中分离絮凝菌。方法:利用马铃薯葡萄糖培养基、麦芽汁琼脂培养基和查氏培养基分离出的丝状菌经絮凝率测定和多次富集筛选得到3株絮凝性能稳定且活性良好的菌株,对各菌株进行形态学鉴定,通过单因素实验和正交试验分析各因素对菌株絮凝能力的影响情况。并对其中一株絮凝率高的菌株SP进行了分子生物学鉴定及絮凝率特性分析。结果:经形态学鉴定SQ为青霉属真菌,SR为丛梗孢科某丝状菌,SP经分子生物学鉴定为草酸青霉菌(Penicillium oxalicum)。SP在麦芽汁培养基,pH 6.0,150 r/min,培养温度28 ℃,摇床培养72 h后对5 g/L高岭土悬浮液的絮凝率可达67.7%。结论:分离出的草酸青霉菌等菌株在微生物絮凝剂研究及水体治理方面具有一定的理论和实际意义。
    Abstract: Objective:To clarify the species and characteristics of filamentous bacteria in sludge,flocculation bacteria were separated from the sewage drain in Jinling Hui Nationality Town,Linzi District,Shandong Province,by means of conventional culture technique. Methods:Three filamentous strains with stable flocculation performance and good activity were obtained by the determination of flocculation rate and multiple enrichment screening of the filamentous bacteria separated with potato glucose medium,wort AGAR medium and chagrin medium. Morphological identification of each strain was carried out. The impacts of each factor on the flocculation ability of the strain were analyzed by single factor experiment and orthogonal test. Molecular biological identification and flocculation rate characteristic analysis were conducted on one strain SP with high flocculation rate. Results:According to the morphological identification,SQ was a penicillium fungus,SR was a filamentous fungus of moniliaceae,and SP was identified as a Penicillium oxalicum by molecular biology. The flocculation rate of SP for 5 g/L kaolin suspension could reach 67.7% after shake cultivation in malt extract medium,pH 6.0,150 r/min and at 28 ℃ for 72 h. Conclusion:The strains such as Penicillium oxalicum separated are of certain theoretical and practical significance in research on microbial flocculant and water treatment.
  • 期刊类型引用(1)

    1. 田瑜琳,雒晓芳,朱雅雯,赵梓彤. 微生物絮凝剂ZF-1C的絮凝效果研究. 生物化工. 2020(03): 90-94 . 百度学术

    其他类型引用(1)

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  • 被引次数: 2
出版历程
  • 收稿日期:  2018-06-25
  • 网络出版日期:  2020-11-12
  • 刊出日期:  2019-10-31

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