Abstract: To investigate the hypoglycemic effect of the inulin compound with Ganoderma polysaccharides,type 2 diabetes mellitus rat model induced by high fat diet and streptozotocin was established. Then the inulin and Ganoderma lucidum polysaccharides were administered orally to the diabetic rats for 5 weeks. During this period,the body weight and fasting blood glucose of T2DM rats(FBG)were measured weekly. Subsequently,the insulin(FINS),glycosylated serum protein(GSP),glutamic acid transaminase(ALT),aspartate aminotransferase(AST),hepatic glycogen and muscle glycogen were also measured,and the HE staining of the liver was observed. The results showed that,compared with the model group,the indicators of FBG,FINS,GSP,ALT and AST of the high combination of inulin and Ganoderma lucidum polysaccharides groups(5 g/kg·bw inulin+0.4 g/kg·bw Ganoderma lucidum polysaccharides)were significantly decreased(P<0.01),the contents of glycogen was significantly increased(P<0.01),and insulin resistance was significantly improved(P<0.01). Moreover,compared with the simple inulin group,all the above indexes in the compound groups were significantly different(P<0.05). In a conclusion,inulin combined with Ganoderma lucidum polysaccharides could enhance synergistic hypoglycemic effect by improving insulin resistance,repairing liver injury and promoting glycogen synthesis.