Abstract: Objective:To develop a method for determination of inositol in food by microbiological method. Methods:In the culture medium without inositol,the growth of inositol deficient strain and the concentration of inositol were positively correlated with each other,which was reached by screening three strains of Saccharomyces uvarum and Saccharomyces cerevisiae,and selecting culture medium. The content of inositol in food was determined by using four-parameter logistic curve method according to absorbance. The methodology of this method had been verified. Results:The experiment had been done by inositol deficient strain-Saccharomyces uvarum(CICC 1465)and commercial culture medium for inositol determination of Qingdao rishui. The curve range of inositol content in natural food and enriched food was 0.1~1.0 μg/mL using this method. The precision,repeatability of this method were fairly good. The recovery was between 90%~110%. The detection limit was 0.375 mg/100 g.Conclusion:The method can accurately determinate inositol content in natural food and enriched food,as well as provide an efficient reference for the establishment and optimization of the microbiological method of inositol in the national food safety standard.