Abstract: The composition of free fatty acids in subcutaneous and intramuscular fat of Xuanwei ham and Jinhua ham was analyzed by gas chromatography-mass spectrometry (GC-MS) for two years of Xuanwei ham and Jinhua ham. The results showed that 22 kinds of free fatty acids were detected in the intramuscular fat and subcutaneous fat of Xuanwei ham and Jinhua ham. The higher fatty acids were palmitic acid (C16:0),stearic acid (C18:0),oleic acid (C18:1n9c) and linoleic acid (C18:2n6c),and there were significant differences in fatty acid composition between different ham and different parts (p<0.05).Total free fatty acid content in intramuscular and subcutaneous fat of Jinhua ham was 29.24,102.68 μg/mg,respectively,which were 34.58% (p<0.05) and 29.09% (p>0.05) higher than those of Xuanwei ham. The content of saturated fatty acids in intramuscular fat of Xuanwei ham and Jinhua ham was higher than that of unsaturated fatty acids,and the content of unsaturated fatty acids in subcutaneous fat was higher than that of saturated fatty acids. The contents of intramuscular and subcutaneous free fatty acids in Jinhua ham were higher than those in Xuanwei ham.