Optimization of Ultrasonic Assisted Extraction of Isovitexin from Zizyphi spinosae Semen by Response Surface Methodology
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摘要: 本研究旨在用响应面方法优化酸枣仁异牡荆素的超声辅助提取工艺。通过单因素实验考察超声时间、超声功率、乙醇体积分数、超声温度和液固比对异牡荆素得率的影响,以这些因素为自变量,异牡荆素得率为响应值,设计四因素三水平Box-Behnken响应面试验方法。结果表明,响应面法优化超声提取条件:超声功率419 W,液固比16:1 mL/g,超声时间61 min,乙醇体积分数73.70%,各因素的影响重要性依次为:乙醇体积分数 > 超声时间 > 液固比 > 超声功率。预测最佳得率0.236%与实际得率0.241%相比,相对误差为2.075%,证明模型理论预测值与实际值拟合效果良好。本研究优化了提取异牡荆素的超声辅助技术,为超声辅助提取黄酮类化合物提供了方法借鉴。
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关键词:
- 酸枣仁 /
- 异牡荆素 /
- 超声辅助提取 /
- 得率 /
- Box-Behnken响应面
Abstract: The ultrasonic assisted extraction of isovitexin from Zizyphi spinosae semen was optimized. On the basis of single factor experiment,ultrasound time,ultrasound power,ethanol volume fraction and liquid-solid ratio were selected as independent variables,the yield of isovitexin was used as the response value. The 4 factors and 3 level response surface tests were designed by Box-Behnken experiment. The results showed that the ultrasonic extraction conditions of optimization by response surface methodology was as follows:Ultrasound power 419 W,liquid-solid ratio 16:1 mL/g,ultrasound time 61 min,ethanol volume fraction 73.70%. The influence factors on the yield of isovitexin was as follows:Ethanol volume fraction > ultrasound time > liquid-solid ratio > ultrasound power. The relative error between the predicted optimum yield 0.236% and the actual yield 0.241% was 2.075%,which proved that the theoretical predicted value of the model fitted well with the actual value. In this study,the ultrasound assisted extraction technology of isovitexin was optimized to provide a reference for ultrasonic-assisted extraction of flavonoids.
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