The Protective Mechanism of Chrysanthemum Chuju against Hypoxia in Rats Depended on HIF-1 and TnT
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摘要: 目的:探讨血清缺氧诱导因子-1(HIF-1)、脂蛋白a(Lp-a)及肌钙蛋白T(TnT)在滁菊抗缺氧保护机制中的作用。 方法:小鼠缺氧暴露前,以DPPH自由基清除率为87%的滁菊提取液0.4 mL进行干预,观察滁菊预处理对缺氧小鼠血清HIF-1、Lp-a及TnT表达的影响;通过比较缺氧耐受小鼠与缺氧敏感小鼠血清HIF-1、Lp-a、TnT表达差异,确定缺氧易感的风险因子及滁菊的耐缺氧保护机制。结果:滁菊干预可促进滁菊干预组缺氧小鼠HIF-1的表达(p<0.01),并下调血清Lp-a、TnT水平(p<0.05);与缺氧敏感组小鼠相比,缺氧耐受小鼠血清中HIF-1水平显著升高(p<0.05),TnT水平极显著降低(p<0.01),Lp-a水平则无统计学差异(p>0.05)。结论:滁菊的耐缺氧保护功能与其上调HIF-1及抑制TnT表达有关。Abstract: Objective:To investigate the role of serum hypoxia-inducible factor-1 (HIF-1), lipoprotein a (Lp-a) and troponin T (TnT) in the anti-hypoxia protective mechanism of Chrysanthemum Chuju. Methods:Before oxygen deprivation, mice were given 0.4 mL extract of Chrysanthemum Chuju with DPPH free radical clearance of 87% to observe the effect of Chrysanthemum Chuju pretreatment on the expression of serum HIF-1, Lp-a and TnT in hypoxic mice. Comparison of differences in serum HIF-1, Lp-a and TnT levels between hypoxia-resistant mice and hypoxia-sensitive mice was carried out to determine the risk factors of susceptibility to hypoxia and the protective mechanism of Chrysanthemum Chuju against hypoxia. Results:Intervention of Chrysanthemum Chuju promoted the expression of HIF-1 in hypoxic mice (p<0.01) and down-regulated the levels of serum Lp-a and TnT (p<0.05). Compared with hypoxia-sensitive mice, the serum level of HIF-1 in hypoxia-tolerant mice was significantly increased (p<0.05) and TnT levels were significantly lower (p<0.01), but no statistical difference found in Lp-a levels (p>0.05). Conclusion:The hypoxia-resistance protective role of Chrysanthemum chuju was related to up-regulation of HIF-1 and inhibition of TnT expression.
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Keywords:
- hypoxia-inducible factor-1 /
- lipoprotein a /
- troponin T /
- hypoxia tolerance /
- Chrysanthemum
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