The Protective Mechanism of Chrysanthemum Chuju against Hypoxia in Rats Depended on HIF-1 and TnT
-
摘要: 目的:探讨血清缺氧诱导因子-1(HIF-1)、脂蛋白a(Lp-a)及肌钙蛋白T(TnT)在滁菊抗缺氧保护机制中的作用。 方法:小鼠缺氧暴露前,以DPPH自由基清除率为87%的滁菊提取液0.4 mL进行干预,观察滁菊预处理对缺氧小鼠血清HIF-1、Lp-a及TnT表达的影响;通过比较缺氧耐受小鼠与缺氧敏感小鼠血清HIF-1、Lp-a、TnT表达差异,确定缺氧易感的风险因子及滁菊的耐缺氧保护机制。结果:滁菊干预可促进滁菊干预组缺氧小鼠HIF-1的表达(p<0.01),并下调血清Lp-a、TnT水平(p<0.05);与缺氧敏感组小鼠相比,缺氧耐受小鼠血清中HIF-1水平显著升高(p<0.05),TnT水平极显著降低(p<0.01),Lp-a水平则无统计学差异(p>0.05)。结论:滁菊的耐缺氧保护功能与其上调HIF-1及抑制TnT表达有关。Abstract: Objective:To investigate the role of serum hypoxia-inducible factor-1 (HIF-1), lipoprotein a (Lp-a) and troponin T (TnT) in the anti-hypoxia protective mechanism of Chrysanthemum Chuju. Methods:Before oxygen deprivation, mice were given 0.4 mL extract of Chrysanthemum Chuju with DPPH free radical clearance of 87% to observe the effect of Chrysanthemum Chuju pretreatment on the expression of serum HIF-1, Lp-a and TnT in hypoxic mice. Comparison of differences in serum HIF-1, Lp-a and TnT levels between hypoxia-resistant mice and hypoxia-sensitive mice was carried out to determine the risk factors of susceptibility to hypoxia and the protective mechanism of Chrysanthemum Chuju against hypoxia. Results:Intervention of Chrysanthemum Chuju promoted the expression of HIF-1 in hypoxic mice (p<0.01) and down-regulated the levels of serum Lp-a and TnT (p<0.05). Compared with hypoxia-sensitive mice, the serum level of HIF-1 in hypoxia-tolerant mice was significantly increased (p<0.05) and TnT levels were significantly lower (p<0.01), but no statistical difference found in Lp-a levels (p>0.05). Conclusion:The hypoxia-resistance protective role of Chrysanthemum chuju was related to up-regulation of HIF-1 and inhibition of TnT expression.
-
Keywords:
- hypoxia-inducible factor-1 /
- lipoprotein a /
- troponin T /
- hypoxia tolerance /
- Chrysanthemum
-
期刊类型引用(8)
1. 林继辉,陈琳榕,冯庆玲,谢三都,戴玉梅. 沙棘果总黄酮提取工艺及抗氧化性研究. 云南民族大学学报(自然科学版). 2024(02): 178-185 . 百度学术
2. 韩冬月,姚妮,王楚茵,方芳,关君. 芦荟大黄素-铝螯合物的合成表征及抗氧化活性研究. 中国现代中药. 2022(03): 452-458 . 百度学术
3. 戢得蓉,刘松奇,熊坤艳,李梦,胡明虎. 雪莲果叶总黄酮超声波辅助酶法提取工艺优化及抗氧化活性研究. 食品与机械. 2021(02): 179-185 . 百度学术
4. 黄九林,彭博,丁瑜瑜,赵小龙. 大黄素-硒(Ⅳ)配合物的合成、表征及抗氧化活性研究. 化学试剂. 2021(07): 992-995 . 百度学术
5. 赵美奇,陈莉,钱现,董晗睿,李晓娜,杜迅. 基于铜离子检测的天然染料变色织物的制备. 纺织导报. 2021(09): 34-36+38 . 百度学术
6. 王勤,申键. 中药金属配合物研究进展. 北方药学. 2021(06): 193-196 . 百度学术
7. 马玲龙,熊亚红,付银莲,陈实,乐学义. 山奈酚-3’, 8-二磺酸钠及其铜(Ⅱ)配合物的制备与抗氧化活性研究. 食品科技. 2020(04): 206-212 . 百度学术
8. 李茜珣,陈永虎,沈家聪,刘翠翠,陈君立,韩丽琴. 红景天黄酮与Mn~(2+)配位反应的动力学研究. 吉林医药学院学报. 2020(06): 411-414 . 百度学术
其他类型引用(1)
计量
- 文章访问数: 183
- HTML全文浏览量: 16
- PDF下载量: 6
- 被引次数: 9