富硒长双歧杆菌DD98菌株的黏附特性及黏附机制初步研究

周艳; 祁艳; 纪瑞; 谭俊; 陈代杰

doi:10.13386/j.issn1002-0306.2019.13.014

富硒长双歧杆菌DD98菌株的黏附特性及黏附机制初步研究

周艳¹,
祁艳²,
纪瑞¹,
谭俊^1,,
陈代杰^1,3,

1. 中国医药工业研究总院, 上海 200040;
2. 复旦大学, 上海 200120;
3. 上海交通大学, 上海 200240

基金项目:

国家青年科学基金项目(81603004)。

详细信息

作者简介:
周艳(1994-),女,硕士研究生,研究方向:微生物与生化药学,E-mail:zhouyan_1994@163.com。

通讯作者:
谭俊(1983-),女,博士,副研究员,研究方向:菌株选育及微生物药物学,E-mail:baiyuge1113@163.com

陈代杰(1957-),男,博士,教授,研究方向:微生物药物研究开发,E-mail:hcb001@163.com。

中图分类号: TS201.3
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出版历程
- 收稿日期: 2018-11-20
- 网络出版日期: 2020-11-12
- 刊出日期: 2019-06-30

Adhesion Ability of Selenium-enriched Bifidobacterium longum DD98 to Caco-2 Cell and Preliminary Study on Its Mechanism

ZHOU Yan¹,
QI Yan²,
JI Rui¹,
TAN Jun^1,,
CHEN Dai-jie^1,3,

1. China State Institute of Pharmaceutical Industry, Shanghai 200040, China;
2. Fudan University, Shanghai 200120, China;
3. Shanghai Jiao Tong University, Shanghai 200240, China

摘要

摘要: 利用Caco-2细胞作为体外黏附模型,通过细菌黏附率评价一株新的长双歧杆菌菌株DD98(Bifidobacterium longum DD98,DD98)富硒前后的黏附能力,测定细菌自动聚集能力与表面疏水性,采用荧光定量PCR技术检测细菌表面Tuf蛋白mRNA表达量差异。结果表明,与DD98相比,富硒DD98(Selenium-enriched Bifidobacterium longum DD98,Se-DD98)黏附于Caco-2细胞的数量从(39.12±17.57)CFU/100 cells上升到(581.17±62.79) CFU/100 cells,Se-DD98对Caco-2细胞的黏附能力极显著升高(p<0.01)。相比DD98,Se-DD98自动聚集能力略有上升,且其表面疏水性从5.12%±0.16%上升到20.25%±2.14%,极显著性升高(p<0.01);荧光定量PCR结果表明,与Caco-2细胞共孵育2 h后,Se-DD98菌体表面Tuf黏附蛋白表达显著上调(p<0.05)。表明DD98经富硒培养后,黏附能力显著提升,其黏附能力的增强可能与细菌表面Tuf黏附蛋白的改变有关。
- 富硒长双歧杆菌DD98 /
- Caco-2细胞 /
- 黏附 /
- Tuf蛋白
Abstract: In order to study whether there was a difference in the adhesion ability of a new Bifidobacterium strain DD98 (Bifidobacterium longum DD98, DD98) before and after selenium enrichment, bacterial adhesion rate was evaluated by in vitro adhesion model of Caco-2 cells, the auto-aggregation ability and surface hydrophobicity of the bacteria were also determined, Tuf mRNA, as the quantitative expression of bacterial surface protein was detected by real-time fluorescent quantitative PCR. The results showed that selenium-enriched Bifidobacterium longum DD98 (Se-DD98) presented extremely higher adhesive ability to Caco-2 cells than DD98 (p<0.01).The average number of DD98 bacteria adhering to Caco-2 cells was (39.12±17.57) CFU/100 cells, while the number of Se-DD98 was (581.17±62.79) CFU/100 cells. Compared with the DD98, the auto-aggregation ability of Se-DD98 increased slightly, and the surface hydrophobicity increased significantly from 5.12%±0.16% to 20.25%±2.14% (p<0.01).The results of real-time PCR showed that the expression of Tuf protein mRNA on Se-DD98 cells significantly up-regulated after 2 h incubation with Caco-2 cells (p<0.05). It was indicated that the adhesion ability of DD98 after selenium-enriched culture was significantly improved, and the enhancement of adhesion might be related to the change of Tuf protein.
- selenium-enriched Bifidobacterium longum DD98 /
- Caco-2 /
- adhesion /
- Tuf protein