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中国精品科技期刊2020
李美萍, 李蓉, 丁鹏霞, 张生万, 郭彩霞. HS-SPME条件优化并结合GC-MS分析新鲜及不同干燥方式香菜的挥发性成分[J]. 食品工业科技, 2019, 40(7): 228-236,247. DOI: 10.13386/j.issn1002-0306.2019.07.039
引用本文: 李美萍, 李蓉, 丁鹏霞, 张生万, 郭彩霞. HS-SPME条件优化并结合GC-MS分析新鲜及不同干燥方式香菜的挥发性成分[J]. 食品工业科技, 2019, 40(7): 228-236,247. DOI: 10.13386/j.issn1002-0306.2019.07.039
LI Mei-ping, LI Rong, DING Peng-xia, ZHANG Sheng-wan, GUO Cai-xia. Optimization of HS-SPME Condition and Analysis of Volatile Compounds in Fresh and Different Drying Coriander by GC-MS[J]. Science and Technology of Food Industry, 2019, 40(7): 228-236,247. DOI: 10.13386/j.issn1002-0306.2019.07.039
Citation: LI Mei-ping, LI Rong, DING Peng-xia, ZHANG Sheng-wan, GUO Cai-xia. Optimization of HS-SPME Condition and Analysis of Volatile Compounds in Fresh and Different Drying Coriander by GC-MS[J]. Science and Technology of Food Industry, 2019, 40(7): 228-236,247. DOI: 10.13386/j.issn1002-0306.2019.07.039

HS-SPME条件优化并结合GC-MS分析新鲜及不同干燥方式香菜的挥发性成分

Optimization of HS-SPME Condition and Analysis of Volatile Compounds in Fresh and Different Drying Coriander by GC-MS

  • 摘要: 目的:比较新鲜和不同干燥方式的香菜挥发性成分,确定香菜的干燥方式。方法:通过单因素实验和正交试验对顶空固相微萃取的条件进行优化,并采用顶空固相微萃取与气相色谱-质谱联用对新鲜和不同干燥方式处理(烘箱干燥、红外干燥、真空干燥、真空冷冻干燥)香菜挥发性成分进行分析。结果:HS-SPME的最优参数为:75 μm DVB/PDMS萃取头,萃取温度70 ℃,样品用量1.00 g,平衡时间30 min,萃取时间40 min,解吸时间4 min。新鲜香菜共鉴定出52种挥发性组分;醛类和醇类为香菜的主要风味成分,包括2-十四烯醛、2-十二烯醛、癸醛、癸醇、(E)-2-癸烯醇、反式-2-十二烯醇、(E)-2-癸烯醛、十二醛、(E)-2-十六烯醛和(E)-2-十三烯醛。四种干燥方式中,红外干燥中检出的醛类和烃类最多,醛类占70%以上,且与新鲜香菜中含量较高的癸醛、2-十二烯醛和2-十四烯醛含量较为接近;真空冷冻干燥在醛类含量上与新鲜香菜相差较大,但其检出的挥发性成分最多;从干燥成本来说,红外干燥所需时间最短,耗能较小,而真空冷冻干燥所需时间长,能耗大,成本较高。结论:综合挥发性成分和干燥成本考虑,可以采取红外干燥的方式干燥香菜。

     

    Abstract: Objective:To compare the volatile compounds of fresh and different drying coriander,and to further determine the drying method. Methods:The conditions of headspace solid-phase microextraction(HS-SPME)were optimized by single factor and orthogonal experiments,and HS-SPME-GC-MS was used to analyze the volatile components of fresh coriander and coriander treated by different drying methods(oven-drying,infrared drying,vacuum-drying,and vacuum freeze-drying). Results:The optimal parameters of HS-SPME were:75 μm DVB/PDMS extraction head,extraction temperature 70 ℃,the amount of sample 1.00 g,equilibration time 30 min,extraction time 40 min,desorption time 4 min. A total of 52 volatile components were identified from fresh coriander;aldehydes and alcohols were the main components of fresh coriander,including 2-tetradecenal,2-dodecenal,decanal,decyl alcohol,(E)-2-decenol,trans-2-dodecenol,(E)-2-decenal,dodecylaldehyde,(E)-2-hexedecenal,and(E)-2-tridecenal. Among the four drying methods,the aldehydes account for more than 70%,and the content of decanal,2-dodecenal and 2-tetradecenal in the fresh coriander was relatively close. The differences between the vacuum freeze-drying and the fresh coriander in the content of aldehydes were larger than others,but the most volatile components were detected by vacuum freeze-drying. In the view of drying cost,the infrared drying took a long time,consumed a lot of energy,and cost more. Conclusion:In accordance with the volatile components and drying costs,infrared drying could be selected to dry coriander.

     

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