摘要:
为了建立一种荧光光谱法测定淀粉中直链淀粉含量的方法,采用荧光光谱法测定了直链淀粉的激发光谱和发射光谱以及直链淀粉与支链淀粉的浓度比对荧光强度的影响。结果表明:直链淀粉的激发波长为370 nm、发射波长为422 nm;在0.10~0.70 μg/mL浓度范围内,直链淀粉荧光强度与其浓度呈良好线性关系,相关系数r=0.9996,检出限为0.026 μg/mL。用该方法测定玉米淀粉和土豆淀粉中直链淀粉的含量,加标回收率为94.5%~121.7%,相对标准偏差(RSD)为2.42%~3.24%。该方法不需加任何生化试剂,不受时间、温度、共存支链淀粉的影响,快速简便,绿色环保,检出限低,结果准确。
Abstract:
In order to establish method for determination of amylose contents in starch by fluorescence spectrometry. The excitation and emission spectra of amylose, and the effect of ratios of amylose to amylopectin on fluorescence intensity were determined by fluorescence spectrometry. The results showed that:The amyloseemitted fluorescence at theexcitation wavelengths of 370 nm and emission wavelengths of 422 nm. In the concentration range of 0.10~0.70 μg/mL, the fluorescence intensity of amylose presented a good linear relationship with its concentration, and the correlation coefficients was 0.9996, and the detection limit was 0.026 μg/mL. The method was applied to the determination of amylose contents in corn starchand potato with recoveries 94.6%~121.7%, and the RSD was 2.42%~3.24%. This method didn't need any biochemical reagents, and had no affected by time, temperature, coexistence of amylopectin, which was fast and simple, green and environmental friendly, low detection limit with accurate results.
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