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中国精品科技期刊2020

HPLC-UV法测定植物甾醇中3种甾醇单体的含量

冯文欢, 吴正章, 张鹏, 常明, 金青哲, 王兴国

冯文欢, 吴正章, 张鹏, 常明, 金青哲, 王兴国. HPLC-UV法测定植物甾醇中3种甾醇单体的含量[J]. 食品工业科技, 2019, 40(5): 244-248. DOI: 10.13386/j.issn1002-0306.2019.05.040
引用本文: 冯文欢, 吴正章, 张鹏, 常明, 金青哲, 王兴国. HPLC-UV法测定植物甾醇中3种甾醇单体的含量[J]. 食品工业科技, 2019, 40(5): 244-248. DOI: 10.13386/j.issn1002-0306.2019.05.040
FENG Wen-huan, WU Zheng-zhang, ZHANG Peng, CHANG Ming, JIN Qing-zhe, WANG Xing-guo. Quantitative Analysis of Three Individual Sterols in Phytosterol by HPLC-UV[J]. Science and Technology of Food Industry, 2019, 40(5): 244-248. DOI: 10.13386/j.issn1002-0306.2019.05.040
Citation: FENG Wen-huan, WU Zheng-zhang, ZHANG Peng, CHANG Ming, JIN Qing-zhe, WANG Xing-guo. Quantitative Analysis of Three Individual Sterols in Phytosterol by HPLC-UV[J]. Science and Technology of Food Industry, 2019, 40(5): 244-248. DOI: 10.13386/j.issn1002-0306.2019.05.040

HPLC-UV法测定植物甾醇中3种甾醇单体的含量

基金项目: 

植物甾醇和植物甾醇酯的行业标准研究(18DZ2200300),上海市2018年度"科技创新行动计划"技术标准项目。

详细信息
    作者简介:

    冯文欢(1994-),男,硕士研究生,研究方向:油脂及植物蛋白工程,E-mail:fengwenhuan666@126.com。

    通讯作者:

    常明(1979-),博士,副教授,研究方向:油脂及植物蛋白工程,E-mail:mingchang@aliyun.com。

  • 中图分类号: TS207.3

Quantitative Analysis of Three Individual Sterols in Phytosterol by HPLC-UV

  • 摘要: 本文建立了一种利用紫外高效液相法(HPLC-UV)测定植物甾醇中菜油甾醇、豆甾醇和β-谷甾醇的方法。采用 Waters Symmetry C18柱(4.6 mm×250 mm,5 μm),以乙腈和水为流动相等度洗脱,柱温30 ℃,流速1.0 mL/min,紫外检测波长为208 nm。实验结果表明:菜油甾醇、豆甾醇和β-谷甾醇在38 min内均能实现基线分离,线性范围分别为2.52~50.30、5.08~101.60、5.10~102.00 μg/mL时,甾醇单体的线性关系良好,相关系数r分别为0.9971、0.9989、0.9991,检测限为2.5 μg/mL;日内精密度在0.06%~3.06%范围内,日间精密度介于1.56%~6.61%;加样回收率介于92.74%~106.25%之间。本方法能够准确测定4种植物甾醇中3种甾醇单体的含量,其中大豆甾醇和木甾醇中3种甾醇单体的含量组成差异较大,大豆甾醇中菜油甾醇和豆甾醇的含量分别为163.80~251.23 mg/g和134.89~235.04 mg/g,远高于木甾醇中的菜油甾醇和豆甾醇含量,而木甾醇中β-谷甾醇含量为(685.10±7.55) mg/g,则明显高于β-谷甾醇在大豆甾醇中的含量。相对现有的高效液相方法,本方法实现了对混合甾醇中菜油甾醇、豆甾醇和β-谷甾醇3种甾醇单体的精确定量分析。
    Abstract: This study was aimed to establish a HPLC approach for the quantitative analysis of campesterol, stigmasterol and β-sitosterol in phytosterol. In HPLC analysis, the chromatographic separation was achieved on a Waters Symmetry C18column (4.6 mm×250 mm, 5 μm) with a mobile phase composed of acetonitrile and water at a flow rate of 1.0 mL/min. In addition, detection wavelength and column temperature were set at 208 nm and 30℃, respectively. The results showed that three sterols reached the baseline separation and the concentration ranges of campsterol, stigmasterol and β-sitosterol were 2.52~50.30, 5.08~101.60 and 5.10~102.00 μg/mL respectively. It indicated a good linear relationship and the correlation coefficients of three sterols was 0.9971, 0.9989, 0.9991.The limit of detection was 2.5 μg/mL, the intra-day precision was between 0.06%~3.06% and the inter-day precision was between 1.56%~6.61%, the recovery of the three components was 92.74%~106.25%. This method could accurately measure campesterol, stigmasterol and β-sitosterol in phytosterols from 4 kinds of production places, the content of 3 individual phytosterols from deodorizer distillate and tall oil had great differences. The content of campesterol and stigmasterol in phytosterol was 163.80~251.23 mg/g and 134.89~235.04 mg/g, much higher than content in wood sterol. The content of β-sitosterol in wood sterol was (685.10±7.55) mg/g, which was higer than phytosterol. It could be concluded that this was a better method for quality assessment of campesterol, stigmasterol and β-sitosterol in phytosterol.
  • 期刊类型引用(5)

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    3. 尹青春,潘永波,邓浩,谭高好,蒋昌顺,邹冬梅. 超高效液相色谱法测定柱花草中4种次级代谢物的含量. 分析试验室. 2021(06): 654-658 . 百度学术
    4. 商云帅,冯梅,王东,杨玉民. HPLC法测定薏米中两种植物甾醇. 保鲜与加工. 2020(04): 197-201 . 百度学术
    5. 陈其钊,林燕纯,刘志鹏,陈嘉敏,李长成,王桂华,李家威,余构彬. 三种糖品中植物甾醇的测定. 食品与发酵工业. 2020(22): 238-244 . 百度学术

    其他类型引用(2)

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  • 被引次数: 7
出版历程
  • 收稿日期:  2018-05-29
  • 网络出版日期:  2020-11-12
  • 刊出日期:  2019-02-28

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