摘要:
目的:分析黔产薏苡仁油成分并研究不同浓度黔产薏苡仁油对破骨细胞活性的影响。方法:采用无水乙醇超声辅助法提取黔产薏苡仁油,并对其进行脂肪酸残基的气相色谱-质谱联用(GC-MS)分析,以雌二醇(10-8 mol/L)作为阳性对照,采用原代分离新西兰乳兔骨髓细胞,并用1α,25-(OH)2Vit D3 诱导获得破骨细胞,通过抗酒石酸酸性磷酸酶(TRAP)+细胞数、TRAP酶活及骨陷窝面积,分析黔产薏苡仁油对破骨细胞活性的影响。结果:通过GC-MS分析黔产薏苡仁油的脂肪酸残基主要为十四烷酸(肉豆蔻酸)、十六烷酸(棕榈酸)、十八烷酸(硬脂酸)、十八碳一烯酸(油酸)、十八碳二烯酸(亚油酸)、二十二烷酸(山嵛酸)、角鲨烯和γ-谷甾醇;进一步实验表明,黔产薏苡仁油各浓度组(0.98、1.95、3.91、7.81、15.63、31.25 μg/mL)对破骨细胞活性具有一定的抑制作用,其中以高浓度组31.25 μg/mL对破骨细胞的活性的抑制作用最为显著(p<0.05),且呈现出较为良好的浓度依赖性。结论:黔产薏苡仁油对破骨细胞活性具有抑制作用。
Abstract:
Objective:To analyze the components of coix seed oil in Guizhou and study the effects of different concentrations of coix seed oil on osteoclast activity. Methods:The ethanol-assisted extraction of coix seed oil in Guizhou was carried out by anhydrous ethanol and the gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid residues was carried out. Estradiol (10-8 mol/L) was used as a positive control. Rabbit bone marrow cells were induced with 1α, 25-(OH)2Vit D3 to obtain osteoclasts. The effects of coix seed oil in Guizhou on osteoclast activity were analyzed by TRAP+cell number, TRAP activity and bone sag area. Results:The fatty acid residues of hazelnut oil produced by GC-MS were mainly myristic acid, palmitic acid, octadecanoic acid (stearic acid), and eighteen carbon monoenoic acid (oleic acid), octadecadienoic acid (linoleic acid), behenic acid, squalene and γ-sitosterol;further experiments showed that the concentration of coix seed oil in Guizhou (0.98, 1.95, 3.91, 7.81, 15.63, 31.25 μg/mL) had a certain inhibitory effect on osteoclast activity, 31.25 μg/mL in the high concentration group inhibited the activity of osteoclasts most significantly (p<0.05), and showed a good concentration dependence.Conclusion:Coix seed oil in Guizhou has an inhibitory effect on osteoclast activity.
百度学术
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