硝普钠对生姜根茎抗氧化酶和苯丙烷途径的影响

李雪; 葛永红; 李灿婴; 段斌; 魏美林; 唐琦; 陈延儒

doi:10.13386/j.issn1002-0306.2019.02.048

硝普钠对生姜根茎抗氧化酶和苯丙烷途径的影响

李雪¹,
葛永红^1,2, ,,
李灿婴^1,2,
段斌¹,
魏美林¹,
唐琦¹,
陈延儒¹

1. 渤海大学食品科学与工程学院, 辽宁锦州 121013;
2. 生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心, 辽宁锦州 121013

基金项目:

辽宁省重点研发计划项目(2017205002)。

详细信息

作者简介:
李雪(1994-),女,硕士研究生,研究方向:果蔬采后生物学与技术,E-mail:1958315685@qq.com。

通讯作者:
葛永红(1979-),男,博士,副教授,研究方向:果蔬采后生物学与技术,E-mail:geyh1979@163.com。

中图分类号: TS255.1
计量
- 文章访问数: 166
- HTML全文浏览量: 22
- PDF下载量: 6
出版历程
- 收稿日期: 2018-04-22
- 网络出版日期: 2020-11-12
- 刊出日期: 2019-01-14

Effect of Sodium Nitroprusside on Antioxidant Enzymes and Phenylpropanoid Pathway of Ginger Rhizomes

1. College of Food Science and Engineering, Bohai University, Jinzhou 121013, China;
2. National&Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou 121013, China

摘要

摘要: 以生姜根茎为试材,研究采后不同浓度硝普钠溶液浸泡处理10 min对其呼吸强度和失重率的影响并筛选最佳浓度,同时分析最佳浓度处理后生姜根茎抗氧化酶活性及苯丙烷途径关键酶活性和产物含量的变化。结果表明,0.25 mmol/L硝普钠处理降低了生姜根茎呼吸强度、抑制了失重率的升高,硝普钠处理还显著提高了生姜根茎过氧化氢含量、超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶的活性。此外,硝普钠处理还提高了苯丙氨酸解氨酶活性、显著提高了4-香豆酰CoA连接酶的活性,促进了木质素、总酚和类黄酮的积累。由此表明,硝普钠处理通过调节生姜根茎抗氧化酶活性和苯丙烷途径延缓其衰老。
- 生姜根茎 /
- 硝普钠 /
- 衰老 /
- 呼吸强度
Abstract: Ginger rhizomes were used as materials to study the effect of sodium nitroprusside (SNP) dipping at different concentration for 10 min on the respiratory intensity and weight loss to screen the optimal concentration of SNP. The changes of antioxidant enzymes activities, key enzymes in phenylpropanoid pathway and metabolites accumulation in ginger rhizomes after SNP treatment were also studied. The results indicated that 0.25 mmol/L SNP treatment decreased the respiratory intensity and inhibited the increase of weight loss. SNP treatment significantly increased the content of hydrogen peroxide (H₂O₂) and the activity of superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX) in ginger rhizomes. The results also showed that SNP treatment enhanced the activity of phenylalanine ammonia-lyase (PAL) and significantly increased 4-coumarate/coenzyme A ligase (4CL) activity to activate the accumulation of lignin, total phenolic compounds and flavonoids in ginger rhizomes. These results suggested that SNP delay senescence of ginger rhizomes by modulating antioxidant enzymes and phenylpropanoid pathway.
- ginger rhizomes /
- sodium nitroprusside /
- senescence /
- respiratory intensity