西藏曲拉和发酵乳中抗氧化和益生特性乳酸菌的筛选及鉴定

刘亚东; 张悦; 贺银凤; 李畅; 吴荣; 贺敏; 李博

doi:10.13386/j.issn1002-0306.2019.02.025

西藏曲拉和发酵乳中抗氧化和益生特性乳酸菌的筛选及鉴定

刘亚东¹,
张悦¹,
贺银凤¹,
李畅¹,
吴荣¹,
贺敏^2, ,,
李博³

1. 内蒙古农业大学食品科学与工程学院, 内蒙古呼和浩特 010018;
2. 内蒙古包头市疾病预防控制中心, 内蒙古包头 014030;
3. 内蒙古伊利实业集团股份有限公司, 内蒙古呼和浩特 010018

基金项目:

内蒙古自然科学基金(2015MS0345)。

详细信息

作者简介:
刘亚东(1993-),男,硕士研究生,研究方向:食品生物技术,E-mail:liuyadong1025@126.com。

通讯作者:
贺敏(1974-),女,本科,主任检验师,研究方向:食品安全与检验,E-mail:1hk1567@163.com。

中图分类号: TS252.1
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出版历程
- 收稿日期: 2018-04-16
- 网络出版日期: 2020-11-12
- 刊出日期: 2019-01-14

Screening and Identification of Lactic Acid Bacteria with Antioxidant and Probiotic Properties from Qula and Fermented Milk in Tibet

1. School of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Baotou Center for Disease Control and Prevention, Baotou 014030, China;
3. Inner Mongolia Yili Industrial Group Limited by Share Ltd., Hohhot 010018, China

摘要

摘要: 以抗过氧化氢、清除自由基为抗氧化指标,耐酸、耐胆盐为益生特性指标,对采集自西藏的自然发酵曲拉和发酵乳中乳酸菌进行了分离、筛选和鉴定。结果表明:从9份样品中分得的62株菌有23株对0.3%过氧化氢具备耐受性,其中4-C、5-D、5-E、9-E对DPPH·和·OH清除率最高分别为86.97%±0.11%、27.62%±0.28%;经鉴定5-D、5-E、9-E、4-C分别为Lactobacillus delbrueckii subsp. Jakobsenii、Lactobacillus delbrueckii subsp. Jakobsenii、Lactobacillus gasseri、Pediococcus pentosaceus;此外,菌株5-D、5-E对酸碱和0.3%胆盐均有较好的耐受能力,具备抗氧化和益生特性,有较大潜力应用于天然抗氧化剂的研究与开发,同时也丰富了西藏地区传统牦牛乳制品中乳酸菌资源库的挖掘工作。
- 西藏 /
- 乳酸菌 /
- 曲拉 /
- 抗氧化活性 /
- 耐酸 /
- 耐胆盐 /
- 鉴定
Abstract: This study isolated and identified lactic acid bacteria from the fermented milk and Qula in Tibet, through antioxidant indicators of anti-hydrogen peroxide and scavenging free radicals and probiotic properties of resistance to acid and bile salt. The results showed that 62 strains of bacteria were acquired, and 23 strains of them were tolerant to 0.3% hydrogen peroxide. Among the 23 strains, 4-C, 5-D, 5-E and 9-E had the highest scavenging capacity of DPPH·and·OH, with the rate of 86.97%±0.11% and 27.62%±0.28% respectively. The strains of 5-D, 5-E, 9-E and 4-C were identified as Lactobacillus delbrueckii subsp. Jakobsenii, Lactobacillus delbrueckii subsp. Jakobsenii, Lactobacillus gasseri, Pediococcus pentosaceus respectively. In addition, the 5-D and 5-E had the capability of concurrently antioxidant and probiotic properties of high tolerance to acid-base and 0.3% bile salt stress. They had enormous potential in the research and development of natural antioxidants, as well as enriched the excavation of lactic acid bacteria resources of traditional yak dairy products in Tibet area.
- Tibet /
- lactic acid bacteria /
- Qula /
- antioxidaant activity /
- acid resistance /
- bile salt resistance /
- identification