市售鲜肉中奇异变形杆菌的ERIC-PCR分型与耐药性分析

孙冷宁; 陈善娇; 王观杏; 毕水莲

doi:10.13386/j.issn1002-0306.2019.02.023

市售鲜肉中奇异变形杆菌的ERIC-PCR分型与耐药性分析

孙冷宁^1,2,
陈善娇^1,2,
王观杏¹,
毕水莲^1, ,

1. 广东药科大学公共卫生学院, 广东广州 510006;
2. 广东药科大学食品科学学院, 广东中山 528458

基金项目:

国家自然科学基金青年科学基金资助项目(31401596)

广东省科技计划项目(2014A040401087、2016A020210132)

广州市珠江科技新星专项资助项目(201710010003)。

详细信息

作者简介:
孙冷宁(1993-),女,硕士研究生,研究方向:食品安全,E-mail:m15975633964_1@163.com。

通讯作者:
毕水莲(1982-),女,博士,副教授,研究方向:食品安全,E-mail:shuilianbi@foxmail.com。

中图分类号: TS201.3
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出版历程
- 收稿日期: 2018-05-14
- 网络出版日期: 2020-11-12
- 刊出日期: 2019-01-14

Analysis of ERIC-PCR Typing and Drug Resistance of Proteus mirabilis in Commercially Available Fresh Meat

1. College of Public Health, Guangdong Pharmaceutical University, Guangzhou 510006, China;
2. College of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, China

摘要

摘要: 目的:研究中山市市售生鲜肉中分离的奇异变形杆菌的同源性和耐药性,分析其基因型与耐药表型之间的关系。方法:采用肠杆菌基因间重复序列(Entrobacter repetive intergenic consensus,ERIC)-PCR分型方法对71株奇异变形杆菌进行分型,所得分型指纹图谱使用Quantity One软件进行处理分析。耐药性检测采用K-B纸片扩散法,参照CLSI制定的标准判读药敏结果。结果:71株奇异变形杆菌的相似性系数为0.64~1.00,以75%为界可分为9个簇。测试的18种抗生素中,对链霉素和红霉素的耐药率最高,均为100%;对青霉素和头孢曲松耐药率最低,分别为16.90%、11.27%。所有菌株均为多重耐药菌,且至少耐6种药物。结论:市售生鲜肉中分离的71株奇异变形杆菌相似性高,耐药情况严重,其基因型与耐药表型之间没有明显的相关性。
- 奇异变形杆菌 /
- 肠杆菌科间重复序列 /
- 基因分型 /
- 耐药性 /
- 鲜肉
Abstract: Objective:To investigate the homology and drug resistance of Proteus mirabilis strains isolated from commercially available fresh meat in Zhongshan city and analyze the relationship between their genotypes and drug resistance phenotypes. Methods:71 P. mirabilis strains were classified by the enterobacterial repetitive intergenic consensus (ERIC) -PCR typing method and the acquired fingerprints were processed with Quantity One software. The Kirby-Bauer disk diffusion method was used for the antimicrobial resistance susceptibility testing, and the results were interpreted according to the standards established by the CLSI. Results:The similarity coefficients of 71 P. mirabilis strains were 0.64~1.00, and they were divided into 9 clusters bounded by 75%. In the 18 antibiotics tested, the strains had the highest rates of resistance to streptomycin and erythromycin, both reached 100%. The resistance rates to penicillin and ceftriaxone were the lowest, 16.90% and 11.27%, respectively. All strains were of multidrug resistance and were resistant to 6 drugs at least. Conclusion:71 P. mirabilis strains isolated from commercial available fresh meat had high similarity and serious drug resistance. There was no obvious correlation between their genotypes and resistance phenotypes.
- Proteus mirabilis /
- entrobacter repetive intergenic consensus(ERIC) /
- genotyping /
- drug resistance /
- fresh meat

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