摘要:
利用双向固体发酵技术,以桑枝和燕麦麸皮为药性基质,探讨了不同碳氮比条件下,韩芝、猴头菌、桑黄和奥德蘑四种药食用真菌的生长情况,并对其发酵后的药性菌质的水溶性及醇溶性成分进行含量测定和抗氧化活性分析。结果表明:随碳氮比的增加,韩芝、奥德蘑菌丝体生长速度未见显著变化,猴头菌与桑黄菌丝体生长速度明显增长(p<0.05)。发酵后混合菌质活性成分含量明显变化,其中当碳氮比为20:1 (g/g)时,桑黄和奥德蘑多糖含量最高分别达到(132.8±1.16) mg/g干物质和(111.22±4.83) mg/g干物质,二者多肽含量明显增加,均增加了一倍多;黄酮含量也有明显增长。多酚整体含量下降,在碳氮比为50:1 (g/g)时,猴头菌、桑黄和奥德蘑的总酚浓度在发酵后分别下降了(6.57±0.18) mg/g干物质、(6.78±0.25) mg/g干物质和(5.64±0.47) mg/g干物质,均下降了约1/3。另外,发酵产物醇提液对DPPH自由基有较强的清除能力,在碳氮比为40:1 (g/g)时,韩芝DPPH自由基清除率最高达到96.90%。四种菌质对于羟自由基的清除作用也有较明显的提升。本文为药食用真菌应用于食品、保健品以及化妆品等方面提供理论依据。
Abstract:
Ganoderma lucidum, Hericium erinaceus, Phellinus igniarius and Oudemasiella radiate were used to prepare medicinal fungal matrix through bi-directional solid fermentation in the medium of mulberry and oat bran under the four different C/N ratios. Then, the growth status of different fungi, the contents of main active substances and the antioxidant activity of the water and ethanol extract of fungal matrix were studied. Results showed that:With the increasing of C/N ratio, there was no significant change in the mycelial growth rate of Ganoderma lucidum and Oudemasiella radiate, and that of Hericium erinaceus and Phellinus igniarius increased significantly (p<0.05). The contents of active constituents of mixed fungi changed obviously. When the ratio of C/N was 20:1 (g/g), the polysaccharides of Phellinus igniarius and Oudemasiella radiate reached (132.8±1.16) and (111.22±4.83) mg/g dry matter. The polypeptide increased obviously of Phellinus igniarius and Oudemasiella radiate increased more than doubled. The flavonoids also increased obviously, but the total polyphenols decreased. When the ratio of C/N was 50:1 (g/g), the total polyphenols of Hericium erinaceus, Phellinus igniarius and Oudemasiella radiate were decreased (6.57±0.18), (6.78±0.25) and (5.64±0.47) mg/g dry matter, a decrease of about 1/3.In addition, the ethanol extract of fermented products had strong scavenging ability on DPPH free radicals, and the highest scavenging rate of Ganoderma lucidum reached 96.90% at 40:1 (g/g) C/N ratio. Four kinds of fungi for the removal ability of hydroxyl radicals also had much more obvious improvement. It aimed to explore more edible and medicinal fungi to apply for food, health products and cosmetics in the future.
百度学术
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