摘要:
采用酸性染料比色法优化燕麦中总生物碱含量的测定方法。以曲尼司特为标准品对照,检测波长为340 nm,对酸性染料种类、缓冲液种类和酸性染料用量进行优化,确定最佳检测条件。结果表明,最佳检测条件为:酸性染料为溴甲酚紫,缓冲液为pH5.4的磷酸二氢钠-氢氧化钠,酸性染料用量为3 mL。经测定,本方法线性范围为0.306~45.9 mg/L,回归方程为y=0.04837x+0.00822,R2=0.9994,平均回收率为94.22%,RSD=1.79%。表明该方法具有良好的线性关系,精密度高,重复性好,且操作简单,可为燕麦总生物碱含量的检测提供参考与依据。
Abstract:
Optimization of the determination conditions of total Avenanthramides in oats by acid dye colorimetry. With the tranilast as a control standard at 340 nm,the acid dye species,buffer solution and acid dye dosage were optimized,and the best detection conditions were determined. The results showed that the best detection conditions were as follows:The acid dye was bromine cresol purple,the buffer was sodium hydrogen phosphate and sodium hydroxide with pH5.4,and the amount of acid dye was 3 mL. The linear range of this method was 0.306~45.9 mg/L,and the regression equation was y=0.04837x+0.00822,R2=0.9994,and the average recovery rate was 94.22%,RSD=1.79%. The results showed that the method had good linear relationship,high accuracy,good repeatability and simple operation,which would provide reference and basis for the determination of total avenanthramides content in oat.
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