Abstract: In order to provide evidence for the selection of fine cultivars,factor analysis method was applied to comprehensively evaluate the nutritional quality of 56 highland barleys from Tibetan plateau. Results showed that 11nutrients in granules from 56 highland barleys were obviously different;The highest difference was observed for total flavonoids,amylose contents relatively least. Through correlation analysis,different correlations among all quality indexes were found depending on cultivar. By factor analysis,6 common factors were extracted,cumulatively contributing to 81.156% of the total variance. Results showed that Qingyong13,Zangqing148,Zangqing690,Zangqing28,Qingyong1476,Zangqing27,Diqing1,Zangqing320,Zangqing16,and Zangqing85 were ranked as the top ten respectively based on comprehensive evaluation of the nutritional quality of highland barleys.